中国农学通报2025，Vol.41Issue(13)：65-70,6.
铁皮石斛'明斛5号'一步成苗技术研究
Study on One-Step Seedling Technology of Dendrobium officinale Variety'Minghu No.5'
摘要
Abstract
The paper aims to simplify the factory seedling rearing procedure of a new variety of Dendrobium officinale'Minghu No.5'and shorten the seedling rearing time.Using stem segment as explants,L9(34)orthogonal test was used to research the effects of hormones(6-BA,NAA),additives(banana puree,activated carbon AC)on the one-step seedling growth of D.officinale'Minghu No.5'.The results showed that the medium suitable for the one-step seedling growth of D.officinale'Minghu No.5'was 1/2MS+6-BA 3 mg/L+NAA 0.5 mg/L+banana puree 50 g/L+AC 1 g/L,after 120 days of inoculation,the proliferation coefficient of buds reached 2.85,and the average plant height of seedlings was 9.00 cm,stem diameter was 6.10 mm,leaf length was 4.50 cm,leaf width was 1.07 cm,leaf number was 5.33,root number was 6.00,root length was 6.70 cm,individual plant weight was 0.78 g.The range analysis results showed that the primary and secondary relationship of the four factors on the proliferation of'Minghu No.5'was 6-BA>NAA>AC>banana puree.The inter-subject effect test showed that the four factors had extremely significant effects on the growth rate,stem segment proliferation coefficient,germination rate and bud proliferation coefficient of one-step seedlings(P<0.01).6-BA was the main factor affecting the growth rate of one-step seedlings,and NAA was the main factor affecting the growth rate of one-step seedlings,stem proliferation coefficient and bud proliferation rate.This study could provide technical reference for factory seedling cultivation of D.officinaleand other new varieties.关键词
铁皮石斛/'明斛5号'/茎段培养/一步成苗/正交设计/6-苄氨基嘌呤/萘乙酸Key words
Dendrobium officinale/'Minghu No.5'/stem culture/one-step seedling/orthogonal design/6-BA/NAA分类
农业科技引用本文复制引用
江金兰..铁皮石斛'明斛5号'一步成苗技术研究[J].中国农学通报,2025,41(13):65-70,6.基金项目
福建省星火项目"优质铁皮石斛新品种'明斛5号'种苗繁育与高效栽培技术示范"(2023S0018). （2023S0018）
