中国畜牧兽医2025，Vol.52Issue(6)：2818-2829,12.DOI:10.16431/j.cnki.1671-7236.2025.06.034
雪白睡莲花粗多糖提取工艺优化及其对金黄色葡萄球菌性小鼠乳腺炎的治疗效果研究
Optimization of the Extraction Process of Crude Polysaccharides from Nymphaea candida and Its Therapeutic Effect on Mouse Mastitis Induced by Staphylococcus aureus
摘要
Abstract
[Objective]The aim of the experiment was to optimize the extraction process of crude polysaccharide from Nymphaea candida and explore its application effect in the treatment of mastitis in mice infected with Staphylococcus aureus.[Method]The experiment used Nymphaea candida as the test material,and the extraction rate of Nymphaea candida crude polysaccharide as the evaluation index,and the one-way factor combined with response surface method was used to optimize its extraction process.At the same time,60 SPF healthy Kunming mice were randomly divided into four groups:Blank control group(BC),model control group(MC),positive control group(PC),and crude polysaccharides from Nymphaea candida(CPNA)group,15 mice in each group.50 μL Staphylococcus aureus with 1 × 104 CFU/mL was injected into mice in each group except for blank control group to construct a mouse mastitis model.After successful modelling,saline,0.13 g/kg ciprofloxacin,and 0.5 g/kg crude polysaccharides from Nymphaea candida were administered by gavage to mice in each group twice a day for 7 d,respectively.Mice in blank control group were not treated.The number of Staphylococcus aureus colonies,the content of tumour necrosis factor-α(TNF-α)and interleukin-6(IL-6)in mammary tissues of mice in each test group were measured at 3,5 and 7 d of administration,respectively,and the pathological histological changes in mammary glands of the mice were also observed to evaluate the therapeutic effect.[Result]Under the conditions of material-liquid ratio was 1∶14,ultrasonic power was 70％,extraction temperature was 57 ℃,extraction time was 30 min,and the number of extraction times was 2,the yield of Nymphaea candida crude polysaccharide was 51.78％.On the 5th and 7th days,the number of colonies in mammary tissue,TNF-α and IL-6 contents of mice in CPNA group showed significantly or extremely significantly lower than that in model control group(P＜0.05 or P＜0.01),and the inflammatory cells and erythrocytes in mammary tissues of mice were significantly reduced.[Conclusion]After optimizing the extraction method using one-factor combined response surface method,the yield of crude polysaccharide from Nymphaea candida was 51.78％.It had a good therapeutic effect on the animal model of mouse mastitis induced by Staphylococcus aureus.The results of this study could provide a scientific basis for the prevention and treatment of clinical mastitis and the development and utilization of Nymphaea candida.关键词
雪白睡莲花/粗多糖/提取工艺/金黄色葡萄球菌/乳腺炎Key words
Nymphaea candida/crude polysaccharide/extraction process/Staphylococcus aureus/mastitis分类
农业科技引用本文复制引用
刘丹丹,罗洁,高函雅,徐李依娜,郝宝山,王馨,张伟..雪白睡莲花粗多糖提取工艺优化及其对金黄色葡萄球菌性小鼠乳腺炎的治疗效果研究[J].中国畜牧兽医,2025,52(6):2818-2829,12.基金项目
牛常见多发病防控关键技术研究与集成示范(2023A02007-2) （2023A02007-2）
国家自然科学基金项目(32460901) （32460901）
2023年新疆自治区青年基金(2023D01B31) （2023D01B31）
中央引导地方科技发展专项资金项目(ZYYD2023C03) （ZYYD2023C03）
"新疆草食动物新药研究与创制"重点实验室2024开放课题(XJCDVM-HDRC-S202420) （XJCDVM-HDRC-S202420）
