中成药2025，Vol.47Issue(6)：1885-1891,7.DOI:10.3969/j.issn.1001-1528.2025.06.017
云南含笑枝叶化学成分及其抗炎活性研究
Chemical constituents from the branches and leaves of Michelia yunnanensis and their anti-inflammatory activities
摘要
Abstract
AIM To study the chemical constituents from the branches and leaves of Michelia yunnanensis Franch.ex Finet & Gagnep.and their anti-inflammatory activities.METHODS The methanol extract was isolated and purified by silica gel,MCI,Sephadex LH-20 and semi-preparative HPLC,then the structures of obtained compounds were identified by physicochemical properties and spectral data.Their anti-inflammatory activities were evaluated by RAW264.7 model.RESULTS Twenty compounds were isolated and identified as dihydrodehydrodiconifenyl alcohol(1),8-hydroxypinoresinol(2),lariciresinol(3),isolariciresinol(4),(7S,8R)-4-hydroxy-3,3',5'-trimethoxy-8',9'-dinor-8,4'-oxyneoligna-7,9-diol-7'-aldehyde(5),thero-2,3-bis-(4-hydroxy-3-methoxypheyl)-3-methoxy-propanol(6),evofolin B(7),(E)-p-coumaryl alcohol γ-O-methyl ether(8),ω-hydroxypropioguaiacone(9),sinapaldehyde(10),isoscopoletin(11),6-hydroxy-5,7-dimethoxycoumarin(12),2α,3α-dihydroxy-2-methylbutyrolactone(13),6-hydroxy-3(1-hydroxy-1-methylethyl)-6-methyl-2-cyclohexen-1-one(14),benzofuran-2-carboxaldehyde(15),3,4-dihydroxy-5-methoxybenzaldehyde(16),3,5-dimethoxy-4-hydroxybenzaldehyde(17),3,4-dihydroxybenzaldehyde(18),3,4-dihydroxybenzoic methyl ester(19),vanillic acid(20).The inhibition rate of compound 1 on NO was 45.39％±0.32％.CONCLUSION Compounds 1-16,18-20 are first isolated from this plant.Compound 1 has anti-inflammatory activity.关键词
云南含笑/枝/叶/化学成分/分离鉴定/抗炎活性Key words
Michelia yunnanensis Franch.ex Finet & Gagnep./branches/leaves/chemical constituents/isolation and identification/anti-inflammatory activities分类
医药卫生引用本文复制引用
沈一帆,郑婷月,王秋桦,李珍全,赵秋叶,宋流东,丁林芬..云南含笑枝叶化学成分及其抗炎活性研究[J].中成药,2025,47(6):1885-1891,7.基金项目
云南省基础研究计划面上项目(202301AT070266) （202301AT070266）
云南省科技厅-昆明医科大学联合专项重点项目(202201AY070001-002) （202201AY070001-002）
云南省高层次人才培养支持计划"教学名师"项目(YNWRJXMS2020052) （YNWRJXMS2020052）
