中成药2025，Vol.47Issue(6)：1892-1900,9.DOI:10.3969/j.issn.1001-1528.2025.06.018
大叶匙羹藤化学成分及其体外降血糖活性研究
Chemical constituents from Gymnema tingens and their in vitro hypoglycemic activity
摘要
Abstract
AIM To study the chemical constituents from Gymnema tingens Spreng.and their in vitro hypoglycemic activity.METHODS The 70％ethanol extract was isolated and purified by macroporous resin,silica gel,sephadex LH-20,and semi-preparative HPLC,then the structures of obtained compounds were identified by physicochemical propeties and spectral data.The in vitro hypoglycemic activity was evaluated by glucose uptake test in L6 cells.RESULTS Seventeen compounds were isolated and identified as 7-desoxyneocynapanogenin A(1),glaucogenin(2),cynatratoside A(3),atratcynoside F(4),(+)-lyoniresinol(5),(+)-lyoniresinol 3-O-α-D-rhamnopyranoside-(1→6)-β-D-glucopyranoside(6),fernandoside(7),3,4-dimethoxy-phenyl-1-O-β-D-apiofuranosyl-(1→2)-β-D-glucopyranoside(8),khaephuoside A(9),khaephuoside B(10),3,4,5-trimethoxy-phenyl-O-β-D-glucopyranoside(11),liquiritigenin(12),7,3'-dihydroxy-flavanone-4'-O-β-D-glucopyranoside(13),pinoresinol(14),syringaldehyde(15),(+)-1-hydroxy-pinoresinol-1-β-D-glucopyranoside(16),β-amyrin(17).Compounds 2-5、7、9、10、12、17 could promote the glucose uptake in L6 cells.CONCLUSION Compound 1 is a new compound,and 2-9、11-13、15-17 are isolated from this plant for the first time.Compounds 2-5、7、9、10、12、17 have good hypoglycemic activity.关键词
大叶匙羹藤/化学成分/分离鉴定/降血糖活性/7-desoxyneocynapanogenin A/C21甾体Key words
Gymnema tingens Spreng./chemical constituents/isolation and identification/hypoglycemic activity/7-desoxyneocynapanogenin A/C21 steroidal glycosides分类
医药卫生引用本文复制引用
刘美余,詹鑫,廖广凤,张进燕,杨新洲,卢汝梅..大叶匙羹藤化学成分及其体外降血糖活性研究[J].中成药,2025,47(6):1892-1900,9.基金项目
国家自然科学基金项目(81860750,82360823) （81860750,82360823）
广西中医药大学桂派中医药传承创新团队项目(2022B005) （2022B005）
