中成药2025，Vol.47Issue(6)：1901-1909,9.DOI:10.3969/j.issn.1001-1528.2025.06.019
毛莲蒿叶绿体基因组结构及其物种鉴定意义
Chloroplast genome structure of Artemisia vestita and its significance for species identification
摘要
Abstract
AIM To sequence the chloroplast genome of Artemisia vestita Wall.ex Bess.METHODS Based on ethnobotanical surveys,sample collection and original plant identification were carried out.The chloroplast genome was sequenced using the Illumina platform,followed by assembly and annotation.A comprehensive comparative analysis was conducted with six Artemisia species.The maximum likelihood(ML)phylogenetic tree was constructed based on the chloroplast genome sequences of A.vestita and 32 other Asteraceae species,with Leptocodon hirsutus D.Y.Hong of Campanulaceae as outgroup.RESULTS The chloroplast genome of A.vestita was 151 204 bp in length,including a small single-copy region of 18 331 bp,a large single-copy region of 82 949 bp,and inverted repeat regions of 24 962 bp,with a total GC content of 37.45％.134 genes were annotated,including 89 protein-coding genes,8 ribosomal RNA genes,and 37 transfer RNA genes.A total of 67 SSRs and 44 LSRs were detected in the chloroplast genome.Comparative analysis with closely related species of Artemisia revealed 3 highly variable genes(clpP,rpl36,ycf1)and 6 highly variable intergenic regions(trnK-UUU-matK,rps18-rpl20,rpl36-infA,rpl14-rpl16,rpl16-rpl3 and trnL-UAG-ccsA),which could serve as candidate DNA barcodes for Artemisia identification.Phylogenetic analysis showed that Artemisia formed a highly supportive monophyletic group,with A.vestita and A.gmelinii Web.ex Stechm.being closely related.CONCLUSION This study may provide fundamental data for phylogenetic analysis of Artemisia,taxonomic identification and DNA barcoding construction of Tibetan herb.关键词
毛莲蒿/藏药/叶绿体基因组/系统发育分析/物种鉴定Key words
Artemisia vestita Wall.ex Bess./Tibetan herb/chloroplast genome/phylogenetic analysis/species identification分类
中医学引用本文复制引用
李晋任,索郎纳甲,赵志礼,米玛卓嘎,尕让甲,倪梁红..毛莲蒿叶绿体基因组结构及其物种鉴定意义[J].中成药,2025,47(6):1901-1909,9.基金项目
国家自然科学基金面上项目(82073959) （82073959）
2020年度西藏自治区藏医药管理局局级科技计划项目(JJKT2020017) （JJKT2020017）
