中国输血杂志2025，Vol.38Issue(6)：833-838,6.DOI:10.13303/j.cjbt.issn.1004-549x.2025.06.014
丙酮酸钠对人体红细胞储存损伤的保护作用及机制研究
Protective effects and mechanisms of sodium pyruvate on storage lesions in human red blood cells
摘要
Abstract
Objective To investigate the protective effects and underlying mechanisms of sodium pyruvate(SP)on RBC storage lesions using an oxidative damage model.Methods Six units of leukocyte-depleted suspended RBCs(discar-ded for non-infectious reasons within three days post-collection)were randomly assigned to four groups:negative control(NS),positive control(PS),experimental group 1(SP1),and experimental group 2(SP2).Oxidative stress was in-duced in the PS group by the addition of hydrogen peroxide(H2O2),while SP1 and SP2 received SP supplementation at different concentrations(25 mM and 50 mM,respectively)in the presence of H2O2.After 1 hour of incubation,RBC mor-phology was assessed microscopically,and biochemical indicators including glutathione(GSH),malondialdehyde(MDA),methemoglobin(MetHb),adenosine triphosphate(ATP),and Na+/K+-ATPase activity were measured.Results RBCs in the PS group exhibited pronounced morphological damage,including cell shrinkage and echinocyte formation,whereas both SP-treated groups showed significantly reduced structural injury.SP treatment led to elevated GSH levels and decreased concentrations of MDA and MetHb,suggesting attenuation of oxidative stress.Additionally,SP enhanced intracellular ATP levels and Na+/K+-ATPase activity,thereby contributing to membrane stability.Notably,the SP2 group(50 mM)demon-strated superior protective effects compared to SP1(25 mM).Conclusion Sodium pyruvate effectively attenuates oxidative storage lesions in RBCs,primarily through its antioxidant properties,energy metabolism supporting ability,and celluar membrane stabilizing function.These findings suggest SP as a promising additive for enhancing the quality and safety of stored RBCs.关键词
红细胞储存损伤/丙酮酸钠/抗氧化/能量代谢/Na+-K+-ATP酶Key words
red blood cell storage lesions/sodium pyruvate/antioxidation/energy metabolism/Na+/K+-ATPase分类
临床医学引用本文复制引用
陈浩宁,李浩,苗琦,高强,孙鑫,梅舜瑜,王丽,连云,罗红林,周晨洁..丙酮酸钠对人体红细胞储存损伤的保护作用及机制研究[J].中国输血杂志,2025,38(6):833-838,6.基金项目
江苏省输血协会英科新创科研基金(JSYK2023029) （JSYK2023029）
