中国输血杂志2025，Vol.38Issue(6)：839-842,4.DOI:10.13303/j.cjbt.issn.1004-549x.2025.06.015
规避抗体对应抗原模式搜寻配合血小板的应用与分析
Application and analysis of compatible platelets matching via antigen avoidance
摘要
Abstract
Objective To investigate the application of antigen avoidance pattern for compatible platelets matching.Methods Samples from two patients with immune-mediated platelet transfusion refractoriness were screened for platelet an-tibodies using solid-phase red blood cell adhesion assay(SPRCA).The genotypes of HLA-A,-B loci were determined via ploymerase chain reaction sequence.The specificity of HLA class I antibodies was detected using Luminex technology.Re-sults Platelet antibody screening via SPRCA yielded positive results in both samples.Antibody specificity testing showed the presence of antibodies against HLA-B65,A80,B13,as well as antibodies against HLA-A11,B52,A24 respectively,with both patients exhibiting 80 kinds of positive antibodies.The antibody avoidance pattern successfully selected compatible platelets for transfusion.The bleeding symptoms of two patients were improved after compatible platelets transfusion.Con-clusion For blood stations with limited platelet gene bank resources,antibody avoidance pattern for compatible platelets matching represents an effective strategy for immune-mediated platelet transfusion refractoriness.关键词
免疫性血小板输注无效/血小板基因库/HLA抗体/配合血小板Key words
immune-mediated platelet transfusion refractoriness/platelet gene bank/HLA antibodies/compatible platelet分类
医药卫生引用本文复制引用
吴少云,郑建勋,江素君,吴世泉..规避抗体对应抗原模式搜寻配合血小板的应用与分析[J].中国输血杂志,2025,38(6):839-842,4.基金项目
衢州市科学技术局指导性科技攻关项目(2021090) （2021090）
浙江省血液安全研究重点实验室开放课题(2019KF004) （2019KF004）
