中国药房2025，Vol.36Issue(12)：1442-1448,7.DOI:10.6039/j.issn.1001-0408.2025.12.05
护肝清脂方饮片汤剂与配方颗粒的化学成分、药效一致性及机制研究
Study on chemical composition,pharmacodynamic consistency and mechanism between Hugan qingzhi formula decoction and its formulated granules
摘要
Abstract
OBJECTIVE To evaluate the contents of characteristic components in Hugan qingzhi formula(HGQZ)decoction and formulated granules and the pharmacodynamic consistency of them on high-fat diet-induced non-alcoholic fatty liver disease(NAFLD)model mice,and explore their potential underlying mechanisms of action.METHODS Liquid chromatography-tandem mass spectrometry was used to analyze and compare the contents of six characteristic components in HGQZ decoction and formulated granules.Male C57BL/6 mice were randomly divided into normal control group,model group,HGQZ decoction low-dose and high-dose groups(13,26 g/kg,calculated by crude drugs),and HGQZ formulated granules low-dose and high-dose groups(13,26 g/kg,calculated by crude drugs),with 6 mice in each group.Except for the normal control group,which was fed a regular diet,the mice in the other groups were fed a high-fat diet for 20 weeks to establish the NAFLD model;at the same time,the mice in each group were gavaged with the corresponding drugs/water once.The fasting blood glucose(FBG)levels,glucose and insulin tolerance,body weight,liver index,white adipose tissue index,brown adipose tissue index,as well as lipid levels(total cholesterol,triglycerides)and liver function indicators(aspartate transaminase,alanine transaminase)were measured.Additionally,histopathological changes and lipid accumulation in liver tissues were observed.The serum samples of mice in the model group,HGQZ decoction high-dose group and HGQZ formulated granules high-dose group were taken for metabolomics analysis,and validation of the underlying mechanisms was conducted.RESULTS There were no statistically significant differences in the contents of ginsenoside Rb1,typhaneoside,isorhamnetin-3-O-neohesperidoside,hyperoside,nuciferine,and 23-acetylalismol B between HGQZ decoction and HGQZ formulated granules(P＞0.05).Compared with the model group,the hepatic histopathological changes in mice were alleviated in both the HGQZ decoction group and all dose groups of HGQZ formulated granules.Inflammatory cell infiltration and lipid vacuoles were reduced.Additionally,there was a general improvement in FBG levels,glucose tolerance,insulin tolerance,body weight,liver index,white/brown adipose tissue index,lipid levels,and liver function indicators(P＜0.05).However,no statistically significant differences were observed between these treatment groups(P＞0.05).There were 234 and 136 differentially expressed serum metabolites identified in the model group versus HGQZ decoction high-dose group,and model group versus HGQZ formulated granules high-dose group,respectively.After taking the intersection,65 common differentially expressed metabolites were obtained,which were enriched in metabolic pathways such as purine metabolism and tricarboxylic acid cycle metabolism.Among these,the content of citrate in the model group was significantly lower than that in both the HGQZ decoction group and HGQZ formulated granules high-dose group(P＜0.05).Both high-dose HGQZ decoction and formulated granules could significantly elevate the phosphorylation levels of AMP-activated protein kinase(AMPK)(P＜0.05).CONCLUSIONS HGQZ decoction and formulated granules contain comparable amounts of characteristic components,and both exhibit equivalent efficacy on NAFLD model mice.The anti-NAFLD effects of HGQZ are associated with the activation of the AMPK energy metabolism pathway.关键词
护肝清脂方/非酒精性脂肪性肝病/饮片汤剂/配方颗粒/一致性研究/含量/药效Key words
Hugan qingzhi formula/non-alcoholic fatty liver disease/decoction/formulated granules/consistency study/content/pharmacodynamics分类
药学引用本文复制引用
张振华,龙昌锐,吴辉星,乡世健,周本杰..护肝清脂方饮片汤剂与配方颗粒的化学成分、药效一致性及机制研究[J].中国药房,2025,36(12):1442-1448,7.基金项目
国家自然科学基金项目(No.82074078) （No.82074078）
广东省基础与应用基础研究基金委员会省企联合基金面上项目(No.2022A1515-220027) （No.2022A1515-220027）
深圳市科技计划项目(No.ZDSYS20220606100801003). （No.ZDSYS20220606100801003）
