中国实验动物学报2025，Vol.33Issue(5)：673-683,11.DOI:10.3969/j.issn.1005-4847.2025.05.006
不同浓度博来霉素诱导特发性肺纤维化小鼠模型的比较研究
Comparative study of idiopathic pulmonary fibrosis in mice induced by bleomycin of different concentrations
摘要
Abstract
Objective To investigate the establishment and evaluation of an idiopathic pulmonary fibrosis(IPF)mouse model induced by the intratracheal infusion of bleomycin(BLM)of different concentrations.Methods Male C57BL/6J mice were randomly divided into a control group,Model-L group(1.5 mg/kg,BLM),Model-M group(2.5 mg/kg,BLM),and Model-H group(3.5 mg/kg,BLM).An IPF mouse model was constructed by one-time intratracheal infusion of BLM.The general status,body mass,survival rate,and lung coefficient of mice in different groups were compared.Pathological changes in lung tissue,the hydroxyproline content,fibrosis markers and inflammatory factor levels were observed.Results Compared with the control group,the survival rate decreased and body weight showed a downward trend in the low-,medium-,and high-dose model groups,with significant increases in lung coefficients.Inflammatory infiltration(P＜0.01)and collagen deposition(P＜0.0001)were observed in the lung tissues of all model groups.Hydroxyproline levels in lung tissue and serum were significantly elevated(P＜0.05).The mRNA levels of fibrosis markers α-Sma,Fn1,and Col1a1 were upregulated(P＜0.001),with significant increases in corresponding protein expression(P＜0.05).The mRNA expression of the inflammatory factor Tgfb1 also increased(P＜0.0001).Conclusion 1.5,2.5 and 3.5 mg/kg BLM can induce an IPF model in C57BL/6J mice.Based on the results observed for survival rate,body mass,lung coefficient changes,lung tissue gross and pathological changes,and fibrosis-related biomarkers,2.5 mg/kg BLM is the optimal concentration for inducing an IPF mouse model.关键词
特发性肺纤维化/博来霉素/小鼠/气管内滴注Key words
idiopathic pulmonary fibrosis/bleomycin/animal model/intratracheal infusion分类
生物学引用本文复制引用
张欣欣,谢铱子,黄慧婷,江勇,李航,廖钢,温武金,詹少锋,黄秀芳..不同浓度博来霉素诱导特发性肺纤维化小鼠模型的比较研究[J].中国实验动物学报,2025,33(5):673-683,11.基金项目
深圳市"医疗卫生三名工程"项目(SZZYSM202206013),广州市科技计划项目(202201020457),国家中医优势专科建设项目(广州中医药大学第一附属医院肺病科),广东省重点科室(中西医协同科室)建设项目,广州中医药大学青年拔尖人才(团队)"揭榜挂帅"项目.Funded by the Sanming Project of Medicine in Shenzhen(SZZYSM202206013),the Guangzhou Science and Technology Project(202201020457),National Chinese Medicine Specialty Construction Project(Department of Lung Disease,the First Affiliated Hospital of Guangzhou University of Chinese Medicine),Guangdong Province Key Department(Chinese and Western Medicine Coordination Department)Construction Project,Guangzhou University of Chinese Medicine Young Top Talents(Team)"Jie Bang Gua Shuai"Project. （SZZYSM202206013）
