中国实验动物学报2025，Vol.33Issue(5)：695-703,9.DOI:10.3969/j.issn.1005-4847.2025.05.008
基于行为实验的睡眠干扰应激诱发焦虑抑郁共患动物模型的建立
Establishment of an animal model of comorbid anxiety and depression induced by sleep interruption stress based on behavioral experiments
摘要
Abstract
Objective This study aimed to investigate the effects of sleep interruption(SI)cycles on emotional behavior in ICR mice,and to establish a mouse model of comorbid anxiety and depression induced by SI.Methods Seventy-two male ICR mice(4～5 weeks old)were divided randomly into a blank group and a model group.Mice in the model group were subjected to SI stress modeling for 1,2,and 3 weeks,respectively.After modeling,emotional behaviors were evaluated using open-field,elevated plus maze,light-dark box,marble-burying,and forced-swimming tests.Serum corticosterone levels were detected by enzyme-linked immunosorbent assay.Results Mice in the model group buried significantly more marbles after 1 week of SI stress,compared with the blank group(P＜0.05).After 2 weeks of stress,mice in the model group also showed a significant decrease in the number of crossings in the light-dark box(P＜0.05)and a significant increase in the number of marbles buried(P＜0.01)compared with the control group.After 3 weeks of stress,mice in the model group showed a significant increase in the number of marbles buried(P＜0.05),a significant decrease in the number of crossings in the light-dark box(P＜0.05),and a significant increase in immobility time in the forced-swim test(P＜0.01).Conclusions ICR mice exhibited significant anxiety-related behaviors after 2 weeks of SI modeling and significant anxiety-and depressive-related behavioral changes after 3 weeks.Three weeks of SI stress can be used to establish a model of comorbid anxiety and depression.关键词
睡眠干扰/焦虑抑郁/动物模型/行为学Key words
sleep interruption/anxiety-depression/animal model/behavior分类
生物学引用本文复制引用
赵永智,姜宁,刘新民,张亦文,罗燕琴,陈芳,许燕飞,潘瑞乐,常琪,刘萌潮,何清湖..基于行为实验的睡眠干扰应激诱发焦虑抑郁共患动物模型的建立[J].中国实验动物学报,2025,33(5):695-703,9.基金项目
中国医学科学院创新工程(2021-I2M-1-034),自治区重大科技专项(2023A02010-3),新疆生产建设兵团NGHJG项目(2023AA503),2024年国家中医药管理局中医药国际合作专项(中心类项目XDZYJZC-001).Funded by the CAMS Innovation Fund for Medical Sciences(2021-I2M-1-034),Major Science and Technology Project in the Autonomous Region(2023A02010-3),NGHJG Project of Xinjiang Production and Construction Corps(2023AA503),2024 International Cooperation Special Project of Traditional Chinese Medicine by the National Administration of Traditional Chinese Medicine(Center Category Project,XDZYJZC-001). （2021-I2M-1-034）
