农学学报2025，Vol.15Issue(6)：1-9,9.
南瓜多糖高通量提取测定体系优化及应用
Optimization and Application of Efficient Detection System for Extraction of Polysaccharides in Pumpkin
摘要
Abstract
To simplify the determination methods of pumpkin polysaccharide content in breeding process,the optimized experiments of phenol sulfuric acid method after hot water extraction of pumpkin polysaccharide were carried out.The detection wavelength and H2SO4 amount were determined by single factor experiment using 23S18 and other pumpkin flesh.The correlation of determination results between unpurified and purified polysaccharide content was clarified.The results showed that the wavelength of pumpkin polysaccharide determined by phenol-sulfuric acid method was 490nm,and the sample in reaction system included:5%phenol:H2SO4 was 2:2:7.The regression equation was established as y=0.0061x+0.1476(R2=0.9965)and the linear relationship was good when the absorbance was in the range of 0.084-3.684.There was a significant correlation between the unpurified and purified polysaccharide content.The polysaccharide contents of 53 pumpkin parents and combinations were determined and analyzed.Two parents(23S2 and 23S14)and two combinations(23FV26 and 23FV5)with high polysaccharide content of more than 100mg/g were selected for breeding.The efficient detection system for polysaccharides was simple to operate,wide in linear range,and the determination results are significantly related to the results of purified polysaccharide,which would improve the efficiency of pumpkin polysaccharide breeding.关键词
南瓜/多糖/单因素试验/苯酚-硫酸法/育种Key words
pumpkin/polysaccharides/single factor experiment/phenol-sulfuric acid method/breeding分类
农业科技引用本文复制引用
宋慧,安学君,黄芸萍,张香琴,吕燕,钱欣欣..南瓜多糖高通量提取测定体系优化及应用[J].农学学报,2025,15(6):1-9,9.基金项目
宁波市公益类科技计划项目"高多糖加工型南瓜分子辅助选育与示范"(2023S087). （2023S087）
