中国实验动物学报2025，Vol.33Issue(5)：721-729,9.DOI:10.3969/j.issn.1005-4847.2025.05.010
基因编辑在肿瘤类器官中的研究进展
Research progress in gene-editing technology in tumor organoids
摘要
Abstract
Organoids have become an important technological platform in cancer research,but simulating the primary tumor tissue structure and function still presents problems.The development of gene-editing technology,especially when combined with tumor organoids,provides a new approach for accurately and comprehensively simulating the in vivo characteristics of tumor models.Introducing specific gene mutations or correcting mutations in tumor organoids through gene-editing technology can allow detailed analysis of the mechanisms of tumor initiation and progression,as well as exploring potential therapeutic targets,accelerating the drug-screening process,and providing new insights for personalized cancer treatment.This article reviews the formation of tumor organoids and the technical aspects of gene-editing strategies,emphasizing their unique applications and prospects in tumor organoids.We also propose that accurately simulating the in vivo microenvironment,promoting the standardization and stability of organoid gene-editing technology,and optimizing the efficiency of gene editing can accelerate the application of organoids in precision medicine research.关键词
基因编辑/肿瘤类器官/CRISPR/Cas9/干细胞Key words
gene-editing/tumor organoids/CRISPR/Cas9/stem cell分类
生物科学引用本文复制引用
李梦瑶,黄敏丽,李鹏,谢俊科,郭梦甜,张永斌,师长宏..基因编辑在肿瘤类器官中的研究进展[J].中国实验动物学报,2025,33(5):721-729,9.基金项目
国家自然科学基金(32070532,32270566).Funded by the National Natural Science Foundation of China(32070532,32270566). （32070532,32270566）
