首页|期刊导航|作物学报（英文版）|MORF1 regulates the editing of orf224 transcript to modulate fertility in the polima cytoplasmic male sterility system of Brassica napus L.

MORF1 regulates the editing of orf224 transcript to modulate fertility in the polima cytoplasmic male sterility system of Brassica napus L.

Benqi Wang Tingdong Fu Bin Yi Lei Chu Huadong Wang Jian Guo Zunaira Farooq Chaozhi Ma Jingxin Tu Jinxiong Shen Jing Wen

作物学报（英文版）2025，Vol.13Issue(3)：840-849,10.

作物学报（英文版）2025，Vol.13Issue(3)：840-849,10.DOI:10.1016/j.cj.2025.03.017

MORF1 regulates the editing of orf224 transcript to modulate fertility in the polima cytoplasmic male sterility system of Brassica napus L.

MORF1 regulates the editing of orf224 transcript to modulate fertility in the polima cytoplasmic male sterility system of Brassica napus L

Benqi Wang ¹Tingdong Fu ²Bin Yi ²Lei Chu ²Huadong Wang ³Jian Guo ²Zunaira Farooq ²Chaozhi Ma ²Jingxin Tu ²Jinxiong Shen ²Jing Wen²

作者信息

1. Institute of Vegetables,Wuhan Academy of Agricultural Sciences,Wuhan 430065,Hubei,China||National Key Laboratory of Crop Genetic Improvement,National Center of Rapeseed Improvement,Hubei Hongshan Laboratory,Huazhong Agricultural University,Wuhan 430070,Hubei,China
2. National Key Laboratory of Crop Genetic Improvement,National Center of Rapeseed Improvement,Hubei Hongshan Laboratory,Huazhong Agricultural University,Wuhan 430070,Hubei,China
3. Key Laboratory of Crop Physiology,Ecology and Genetic Breeding,Agronomy College,Jiangxi Agricultural University,Nanchang 330045,Jiangxi,China
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摘要

Abstract

The Brassica polima cytoplasmic male sterility(pol CMS)line causes complete and stable sterility and is most extensively used in breeding.The regulatory pathway,however,is not clear.In this work,we stud-ied molecular interaction among several key genes involved in pol CMS.Firstly,we found that the mul-ticellular organelle RNA-editing factor protein(Bna.MORF1)interacted with the pol CMS-restorer protein RFP using the yeast two-hybrid system.Secondly,knock down of Bna.MORF1 using CRISPR/Cas9 editing resulted in sterile transgenic lines.The function of the pol CMS sterility gene orf224 was further confirmed by ectopic expression of the gene in both Arabidopsis and Brassica.Furthermore,using CRISPR/Cas9 we determined that an anther-specific proline-rich protein(APG)was also involved in sterility.We propose a working model for pol CMS in Brassica napus that may expedite the utilization of this popular CMS line in Brassica breeding.

关键词

Polima CMS/Rfp/Bna.MORF1/Bna.APG

Key words

Polima CMS/Rfp/Bna.MORF1/Bna.APG

引用本文复制引用

Benqi Wang,Tingdong Fu,Bin Yi,Lei Chu,Huadong Wang,Jian Guo,Zunaira Farooq,Chaozhi Ma,Jingxin Tu,Jinxiong Shen,Jing Wen..MORF1 regulates the editing of orf224 transcript to modulate fertility in the polima cytoplasmic male sterility system of Brassica napus L.[J].作物学报（英文版）,2025,13(3):840-849,10.

基金项目

We are grateful to Prof.Qijun Chen for providing us with CRISPR/Cas9 vectors and the National Oilseed Rape Center for pro-viding Brassica napus seeds.This work was supported by the Wuhan Science and Technology Major Project on Key Techniques of Biological Breeding and Breeding of New Varieties(2022021302024851),the National Key Research and Develop-ment Program of China(2021YFD1600500),and the National Nat-ural Science Foundation of China(32201883). （2022021302024851）

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