中国比较医学杂志2025，Vol.35Issue(5)：51-59,9.DOI:10.3969/j.issn.1671-7856.2025.05.005
三种卵巢储备功能减退小鼠模型的效果比较
Comparison of three methods for induction of model mice with diminished ovarian reserve
摘要
Abstract
Objective To compare the effects of Tripterygium wilfordii polyglycosides,cyclophosphamide,and cisplatin on the establishment of a mouse model of diminished ovarian reserve(DOR).Methods Mice were randomly divided into the following treatment groups:control(Ctrl),Tripterygium wilfordii polyglycosides(TWP),cyclophosphamide(CTX),and cisplatin(DDP).Mice in the TWP group received a 50 mg/kg suspension of Tripterygium wilfordii polyglycosides by gavage for 14 days,mice in the CTX group received a 20 mg/kg cyclophosphamide suspension by intraperitoneal injection for 14 days,and mice in the DDP group received a 1.5 mg/kg cisplatin solution by intraperitoneal injection for 14 days.The body weight,uterine index,and ovarian index were recorded,the estrous cycle was monitored using the vaginal smear method,and the levels of anti-Mullerian hormone(AMH),estradiol(E2),follicle stimulating hormone(FSH),and luteinizing hormone(LH)were detected using ELISA.Hematoxylin and eosin staining was used to detect ovarian follicle development.The rates of oocyte maturation and fertility were analyzed.Results The three treatment groups of mice all showed the following:significantly decreased body weight and ovarian index(P＜0.05);apparent disorder of the estrous cycle;significantly decreased levels of AMH and E2(P＜0.05);decreased and increased rates of developing follicles and atretic follicles,respectively(P＜0.05);and significantly decreased rates of oocyte maturation,pregnancy,and live birth(P＜0.05).Conclusions DOR mouse models were successfully constructed using Tripterygium wilfordii polyglycosides,cyclophosphamide,or cisplatin,as evidenced by decreased body weight and ovarian index,disordered estrous cycle and hormones,and DOR function,resulting in reduced rates of oocyte maturation,pregnancy,and total number of live births.These DOR effects were most appropriate in the cyclophosphamide group.关键词
卵巢储备功能减退/雷公藤多苷/环磷酰胺/顺铂/动物模型Key words
diminished ovarian reserve/Tripterygium wilfordii polyglycosides/cyclophosphamide/cisplatin/animal model引用本文复制引用
林华丹,王旭,陈春宏,韦郁卓,罗艳春,庞方惠,秦秋云,阮子芸..三种卵巢储备功能减退小鼠模型的效果比较[J].中国比较医学杂志,2025,35(5):51-59,9.基金项目
广西科技厅自然科学基金(2024GXNSFAA010394,2025GXNSFAA069653) （2024GXNSFAA010394,2025GXNSFAA069653）
广西中医药大学"岐黄工程"高层次人才团队培育项目(202401) （202401）
广西壮族自治区妇幼保健院重点实验室开放课题(GXWCHZDKF-2022-07) （GXWCHZDKF-2022-07）
广西研究生教育创新计划项目(YCSW2024414,YCSY2025004) （YCSW2024414,YCSY2025004）
广西中医药大学大学生创新创业项目(202410600005). （202410600005）
