组织工程与重建外科杂志2025，Vol.21Issue(3)：230-237,8.DOI:10.3969/j.issn.1673-0364.2025.03.004
鼠尾草酚抑制增生性瘢痕形成的实验研究
Carnosol inhibits the formation of hypertrophic scar
摘要
Abstract
Objective Hypertrophic scar(HS)is a common fibrotic skin disorder characterized by the activation of fibroblast and the excessive deposition of extracellular matrix(ECM).This study intends to explore the inhibitory effect of carnosol on HS.Methods Human hypertrophic scar-derived fibroblasts(sHSFs)were treated with 5-80 μmol/L carnosol,and CCK-8 method was used to confirm the appropriate concentration of carnosol.Then HSFs were treated with 30 μmol/L carnosol and wound healing assay was used to detect migration ability of HSFs.Moreover,HSFs were treated with 10-30 μmol/L carnosol,qRT-PCR and western blot(WB)were used to evaluate the effects of carnosol on mRNA and protein expression of collagen secretion and activation-related genes in HSFs.Rabbit ear scar model was established and treated with 10 μmol/L or 30 μmol/L carnosol until 14th day after surgery,on the 14th and 28th day after the establishment of model,samples were collected for HE and Masson's trichrome staining to evaluate tissue structure,and α-SMA immunohistochemical staining was used to evaluate the fibrosis degree of tissue.Results Carnosol could inhibit the proliferation of HSFs in a dose-dependent manner.30 μmol/L carnosol treatment could significantly inhibit the migration of HSFs.And carnosol could inhibit the collagen secretion and activation of HSFs in a dose-dependent manner.The rabbit ear scar model showed that carnosol could significantly inhibit the formation of hypertrophic scar,manifested as decreased fibrosis degree and decreased expression of α-SMA.Conclusion Experiments have confirmed that carnosol can significantly inhibit the formation of hypertrophic scar both in vivo and in vitro,and its potential mechanism may be related to the effect of carnosol on fibroblast function.关键词
鼠尾草酚/成纤维细胞/增生性瘢痕Key words
Carnosol/Fibroblasts/Hypertrophic scar分类
临床医学引用本文复制引用
徐玮,吕昊,薛雅心,赵丹阳,许佳,韩冬..鼠尾草酚抑制增生性瘢痕形成的实验研究[J].组织工程与重建外科杂志,2025,21(3):230-237,8.基金项目
上海交通大学医学院附属第九人民医院"交叉"研究基金项目(JYJC202236). （JYJC202236）
