植物遗传资源学报2025，Vol.26Issue(6)：1091-1105,15.DOI:10.13430/j.cnki.jpgr.20240626003
甘薯PAL基因家族的全基因组鉴定及生物信息学分析
Genome-wide Identification and Bioinformatics Analysis of the PAL Gene Family in Sweetpotato[Ipomoea batatas(L.)Lam.]
摘要
Abstract
Phenylalanin ammonia-lyase(PAL)is a key and rate-limiting enzyme in the phenylpropane metabolic pathway,which plays an important role in plant growth,development and stress tolerance.In this study,we screened PAL gene family members in the whole genome of sweetpotato,and the physicochemical properties,phylogeny,conserved domains,chromosomal localization and gene expression of sweetpotato PAL gene family members were analyzed.The results showed that a total of 33 IbPAL genes were identified in the whole genome of sweetpotato.These genes were unevenly distributed on chromosomes 2,6,9 and 15.PAL proteins have small differences in physicochemical properties and similar conserved motifs and gene structures.Multiple cis-acting elements related to abiotic and biotic stress response,phytohormone responsive and growth and development regulation are present on the promoter of IbPAL.There were collinear relationships between sweetpotato IbPAL and Arabidopsis thaliana,Ipomoea trifida and Ipomoea triloba PAL genes.The transcriptome data of sweetpotato showed that the expression of IbPAL gene in sweetpotato was tissue-specific,and the expression of IbPAL19,IbPAL13 and IbPAL7 in storage root was significantly higher than that in undifferentiated root and fibrous roots.The results of qRT-PCR showed that IbPAL gene was induced by drought and salt stress,and the expression patterns of IbPAL were different under different stress treatments.This study provides a reference for further understanding the function of PAL family gene in sweetpotato and lays a foundation for genetic improvement of sweetpotato.关键词
甘薯/PAL基因家族/全基因组鉴定/生物信息学分析Key words
sweetpotato/PAL gene family/complete genome identification/bioinformatics analysis引用本文复制引用
王崇,兰孟焦,肖满秋,潘皓,邓佳祺,吴问胜..甘薯PAL基因家族的全基因组鉴定及生物信息学分析[J].植物遗传资源学报,2025,26(6):1091-1105,15.基金项目
国家现代农业产业技术体系项目(CARS-10-C8) （CARS-10-C8）
江西省农作物良种联合攻关项目(9021109126) （9021109126）
江西省农业科学院基础研究与人才培养专项(JXSNKYJCRC202422)National Modern Agricultural Industry Technology System(CARS-10-C8) （JXSNKYJCRC202422）
Jiangxi Joint Research Project on Crop Cultivar Breeding(9021109126) （9021109126）
Basic Research and Talent Training Project of Jiangxi Academy of Agricultural Sciences(JXSNKYJCRC202422) （JXSNKYJCRC202422）
