甘薯IbSAUR76基因表达特征及互作蛋白筛选
Identification of IbSAUR76 Gene Expression Profiles and Screening of Interacting Proteins in Sweetpotato
摘要
Abstract
Sweetpotato[Ipomoea batatas(L.)Lam.]is susceptible to various biotic and abiotic stresses that adversely affect its yield and quality.Therefore,identifying stress-responsive genes and elucidating their functional mechanisms are crucial for developing stress-resistant cultivars.As key components of auxin signaling pathways,SAUR(small auxin up RNA)genes play pivotal roles in plant growth,development,and stress adaptation.In this study,the IbSAUR76 gene was cloned and its encoded protein was demonstrated to localize to the nucleus and membrane via transient expression in tobacco.Tissue-specific expression profiling demonstrated that IbSAUR76 is ubiquitously expressed in roots,stems,leaves,petioles,and shoot tips(highest in shoot tips),as well as in storage roots at 60,75,90,and 105 days post-planting(peak at 90 days).Analysis of the IbSAUR76 promoter sequence revealed the presence of multiple cis-acting elements associated with stress and hormone responses in the upstream region of the gene.qRT-PCR analysis demonstrated that IbSAUR76 responds to both drought and salt stress treatments.To elucidate the molecular mechanisms underlying its function in sweetpotato,the IbSAUR76 bait vector was constructed for yeast two-hybrid screening against a sweetpotato cDNA library using the mating method,leading to the identification of 27 candidate interacting proteins.Two interactors IbERF2 and IbERF15,which are known to be associated with abiotic stress responses,were further validated through yeast two-hybrid reciprocal verification assays.This study enhances the understanding of the IbSAUR76 gene function and provides a theoretical foundation for in-depth dissection of its role and mechanism of action in sweetpotato.关键词
甘薯/IbSAUR76/生长素/互作蛋白Key words
sweetpotato/IbSAUR76/auxin/interacting protein引用本文复制引用
白楠楠,陈伟,贺立恒,贾小云,曹艳东,郑亚婷,罗状,赵彩良,唐锐敏,吴宇浩,武小平,贾峥嵘..甘薯IbSAUR76基因表达特征及互作蛋白筛选[J].植物遗传资源学报,2025,26(6):1106-1118,13.基金项目
中央引导地方科技发展资金项目(YDZJSX2024B007) （YDZJSX2024B007）
山西农业大学科技创新提升工程(CXGC2023051,CXGC2023074) （CXGC2023051,CXGC2023074）
山西省回国留学人员科研资助项目(2021-074) （2021-074）
忻州市科学技术局项目(20220703) （20220703）
山西省基础研究计划(202403021212098) （202403021212098）
山西农业大学杂粮研究院项目(Z120220502)The Central Guidance for Local Science and Technology Development Fund Project(YDZJSX2024B007) （Z120220502）
Science and Technology Innovation Enhancement Programs of Shanxi Agricultural University(CXGC2023051,CXGC2023074) （CXGC2023051,CXGC2023074）
Research Funding Project for Returned Overseas Students in Shanxi Province(2021-074) （2021-074）
Projects of Xinzhou Municipal Science and Technology Bureau(20220703) （20220703）
Shanxi Province Basic Research Program(202403021212098) （202403021212098）
Projects of the Millet Research Institute of Shanxi Agricultural University(Z120220502) （Z120220502）
