中国中医药信息杂志2025，Vol.32Issue(7)：34-41,8.DOI:10.19879/j.cnki.1005-5304.202411688
基于网络药理学结合动物实验从AMPK信号通路探讨二陈汤加减防治高脂血症痰湿证作用机制
Exploration on the Mechanism of Modified Erchen Decoction in Preventing and Treating Hyperlipidemia with Phlegm-Dampness Syndrome through AMPK Signaling Pathway Based on Network Pharmacology and Animal Experiment
摘要
Abstract
Objective To explore the mechanism of the modified Erchen Decoction in preventing and treating hyperlipidemia with phlegm-dampness syndrome based on network pharmacology and animal experiment.Methods Active components and potential targets of modified Erchen Decoction were screened using TCMSP database.Hyperlipidemia disease targets were retrieved from GeneCards,DrugBank,DisGeNET,OMIM and TTD databases,and the intersection of drugs and disease targets was taken.A protein-protein interaction network was constructed using STRING database,and GO and KEGG pathway enrichment analysis on intersecting targets was conducted using DAVID database.Cytoscape 3.9.1 software constructed a component-target-pathway network to analyze key pathways.The molecular docking between main active components and key targets was performed.A rat model of hyperlipidemia with phlegm dampness syndrome was prepared,and was intervened with modified Erchen Decoction.Liver index was calculated,lipid levels were detected,liver tissue morphology was observed by HE staining,and related protein expressions in liver tissue were detected by Western blot.Results Network pharmacology analysis showed that there were 41 targets of modified Erchen Decoction in preventing and treating hyperlipidemia,including HMGCR,SREBF1,FASN,etc.,involved in AMPK signaling pathway,lipid and atherosclerosis,chemical carcinogenesis-receptor activation,etc.The animal experiment results showed that modified Erchen Decoction could effectively regulate the blood lipid levels of model rats,reduce liver fat accumulation,significantly increase p-AMPK protein expression in liver tissue(P<0.05),and reduce HMGCR,SREBP1c and FAS protein expressions(P<0.05).Conclusion Modified Erchen Decoction may inhibit the synthesis and absorption of cholesterol and fatty acids by activating AMPK/SREBP1c signaling pathway,so as to improve hyperlipidemia.关键词
高脂血症/二陈汤/AMPK信号通路/网络药理学/作用机制Key words
hyperlipidemia/Erchen Decoction/AMPK signaling pathway/network pharmacology/mechanism分类
医药卫生引用本文复制引用
周琴宜,陶志娟,王萍,程绍民,单思,张璋..基于网络药理学结合动物实验从AMPK信号通路探讨二陈汤加减防治高脂血症痰湿证作用机制[J].中国中医药信息杂志,2025,32(7):34-41,8.基金项目
国家自然科学基金(81960851) （81960851）
江西省中医药管理局中医证候基础重点研究室(2022年) （2022年）
江西省卫生计生委中医药科研计划项目(2017A252) （2017A252）
江西中医药大学中医体质-状态辨识健康管理研究团队项目(CXTD22016) （CXTD22016）
江西中医药大学博士科研启动基金课题(2021WBZR001) （2021WBZR001）
