甘薯IbGAPCp1在干旱和盐胁迫下的表达及上游调控因子筛选
Expression Profiles of Sweetpotato IbGAPCp1 under Drought and Salt Stress Conditions and Identification of Its Upstream Regulatory Factors
摘要
Abstract
Plastid glyceraldehyde-3-phosphate dehydrogenase(GAPCp)plays an important role in plant growth and development,energy metabolism,and stress response.In this study,we cloned the open reading frame(ORF)sequence of the IbGAPCp1 gene from sweetpotato variety'Lizixiang'.Subcellular localization studies showed that the IbGAPCp1 protein is targeted to the chloroplasts.Quantitative real-time PCR analysis showed that IbGAPCp1 is ubiquitously expressed across sweetpotato tissues,with the highest transcripts detected in leaves.The expression of IbGAPCp1 gene increased gradually with the development of sweetpotato tuberous root and reaching its peak at 105 days.In order to further explore the regulatory mechanism of IbGAPCp1 gene,we cloned a 1940 bp sequence of the IbGAPCp1 gene promoter,designated as Pro-IbGAPCp1.The prediction of cis-acting elements showed that Pro-IbGAPCp1 contains several elements related to photoresponse,hormone response and meristem expression.Expression profiling under drought and salt stress showed that IbGAPCp1 is responsive to both drought and salt stresses.Furthermore,yeast one-hybrid screening identified 35 upstream transcriptional regulators interacting with the promoter region of IbGAPCp1,including ethylene-insensitive protein 2,zinc finger CCCH domain-containing protein 47 and MYB44,factors that are known to modulate plant growth and development,secondary metabolism,energy production,and stress responses.This study provides a theoretical basis for future elucidating the functional role of IbGAPCp1 in sweetpotato abiotic stress tolerance.关键词
甘薯/质体甘油醛-3-磷酸脱氢酶/酵母单杂交/非生物胁迫Key words
sweetpotato/plastid glyceraldehyde-3-phosphate dehydrogenase/yeast one-hybrid/abiotic stress引用本文复制引用
曹艳东,陈伟,贺立恒,贾小云,白楠楠,罗状,郑亚婷,赵彩良,唐锐敏,武小平,贾峥嵘,吴宇浩..甘薯IbGAPCp1在干旱和盐胁迫下的表达及上游调控因子筛选[J].植物遗传资源学报,2025,26(6):1119-1131,13.基金项目
山西省研究生科研创新项目(2023KY321) （2023KY321）
中央引导地方科技发展资金项目(YDZJSX2024B007) （YDZJSX2024B007）
山西农业大学科技创新提升工程(CXGC2023051,CXGC2023074) （CXGC2023051,CXGC2023074）
山西省回国留学人员科研资助项目(2021-074) （2021-074）
山西省基础研究计划(202403021212098) （202403021212098）
山西农业大学杂粮研究院项目(Z120220502,ZLZD2404)The Graduate Research and Innovation Projects of Shanxi Province(2023KY321) （Z120220502,ZLZD2404）
The Central Guidance for Local Science and Technology Development Fund Project(YDZJSX2024B007) （YDZJSX2024B007）
Science and Technology Innovation Enhancement Programs of Shanxi Agricultural University(CXGC2023051,CXGC2023074) （CXGC2023051,CXGC2023074）
Research Funding Project for Returned Overseas Students in Shanxi Province(2021-074) （2021-074）
Shanxi Province Basic Research Program(202403021212098) （202403021212098）
Projects of the Millet Research Institute of Shanxi Agricultural University(Z120220502,ZLZD2404) （Z120220502,ZLZD2404）
