中成药2025，Vol.47Issue(6)：1825-1833,9.DOI:10.3969/j.issn.1001-1528.2025.06.008
石菖蒲挥发油对抽动障碍大鼠CX3CL1/CX3CR1信号轴及神经炎症的影响
Effects of volatile oil from Acorus tatarinowii on CX3CL1/CX3CR1 signal axis and neuroinflammation in a rat model of tic disorders
摘要
Abstract
AIM To investigate the effects of volatile oil from Acorus tatarinowii Schott(A.tatarinowii)on neuroinflammation in a rat model of tic disorders.METHODS The SD rats were randomly divided into the blank group(8 rats)and the model group(40 rats).The rat models of tic disorders established successfully by intraperitoneal injection of iminodiapropionitrile(IDPN)were further divided into the model group,the tiapride group and the high-dose,moderate-dose and low-dose A.tatarinowii volatile oil groups,with 8 rats in each group.The 4-week intragastric treatment of respective drug was initiated the next day after the completion of modeling,and normal saline was dosed upon the blank group and the model group,during which the rats' behavioral changes were assessed by stereotyped behavior and motor behavior score every week.After the administration,the rats had their morphological changes of striatal neurons observed by Nissl staining;their levels of TGF-β,IL-10,TNF-αand IL-1β in serum and striatum detected by ELISA;their striatal protein expressions of CX3CL1 and CX3CR1 detected by Western blot and immunohistochemistry;and their striatal expressions of M1,M2 microglia marker proteins CD86,CD206,SYN and PSD-95 detected by immunofluorescence co-staining.RESULTS Compared with the model group,the A.tatarinowii volatile oil groups demonstrated improved twitch-like behavior;decreased scores of motor behavior and rigid behavior(P＜0.01);alleviated damage of Nissl bodies in neurons;increased serum and striatum levels of TGF-β and IL-10(P＜0.05,P＜0.01);decreased levels of TNF-α and IL-1β(P＜0.01);decreased striatal protein expressions of CX3CL1 and CX3CR1(P＜0.01);increased protein expressions of PSD95 and SYN(P＜0.05,P＜0.01);and decreased CD86/Iba1(P＜0.01)and increased CD206/Iba1(P＜0.01)in terms of the fluorescence intensity.CONCLUSION A.tatarinowii volatile oil contributes an anti-tic effect and improves the neuroinflammation in the brain of the rat model of tic disorders by promoting the transformation of microglia into M2 type via CX3CL1/CX3CR1 signal axis.关键词
石菖蒲挥发油/抽动障碍/小胶质细胞/神经炎症/突触蛋白/CX3CL1/CX3CR1信号轴Key words
Acorus gramineus volatile oil/tic disorders/microglial cells/neuroinflammation/synaptic protein/CX3CL1/CX3CR1 signal axis分类
医药卫生引用本文复制引用
丁艳琴,逯若兰,李玲,冯鹏,王明露,王宇彤,孙可馨,魏星,田永衍,汤兴萍,李萍..石菖蒲挥发油对抽动障碍大鼠CX3CL1/CX3CR1信号轴及神经炎症的影响[J].中成药,2025,47(6):1825-1833,9.基金项目
国家自然科学基金地区项目(82460953) （82460953）
甘肃省科技计划项目(22JR11RG221) （22JR11RG221）
甘肃省中医药管理局科研课题(GZKP-2023-55) （GZKP-2023-55）
甘肃省高等学校青年博士基金项目(2023QB-075) （2023QB-075）
河西学院校长基金青年科研项目(QN2023001) （QN2023001）
河西学院博士科研启动经费项目(KYQD2022015) （KYQD2022015）
河西学院大学生科技创新项目(2023-123,2024-105) （2023-123,2024-105）
大学生创新创业训练计划项目(S202310740011,202410740035) （S202310740011,202410740035）
