中国中医药信息杂志2025，Vol.32Issue(7)：73-80,8.DOI:10.19879/j.cnki.1005-5304.202411104
基于EP3-HCN1通路探讨电针对骶上脊髓损伤后尿失禁大鼠的效应机制
Exploration on the Effect Mechanism of Electroacupuncture in Treating Urinary Incontinence Rats after Suprasacral Spinal Cord Injury Based on EP3-HCN1 Signaling Pathway
摘要
Abstract
Objective To explore the mechanism of electroacupuncture in treating rats with urinary incontinence after suprasacral spinal cord injury based on EP3-HCN1 signaling pathway.Methods Totally 48 female SD rats were divided into sham-operation group(12 rats)and modeling group(36 rats,prepared as urinary incontinence model rats after suprasacral spinal cord injury)according to the random number table method.The molded rats were randomly divided into the model group,electroacupuncture group and electroacupuncture+EP3 agonist group after successful modeling,with 11 rats in each group.The electroacupuncture group stimulated"Guanyuan","Zhongji"and"Sanyinjiao"acupoints,and the electroacupuncture+EP3 agonist group injected EP3 agonist sulprostone into the tail vein before electroacupuncture treatment once a day for 7 days.The urodynamic indexes of rats were detected,the morphology of the bladder tissues were observed by HE staining,Western blot and RT-qPCR were used to detect the protein and mRNA expressions of c-Kit,EP3 and hyperpolarized activated cyclic nucleotide gated channel protein(HCN)1 in bladder tissue,respectively.Immunofluorescence was used to detect the positive expressions of c-Kit,EP3 and HCN1 in bladder tissue.Results Compared with the sham-operation group,the model group rats showed an increase in bladder basal pressure and leak point pressure(P<0.01),and a decrease in bladder maximum capacity and compliance(P<0.01);the arrangement of epithelial cells in bladder tissue was disordered,leading to pathological phenomena such as inflammatory cell infiltration,tissue edema and bleeding;the protein and mRNA expressions of c-Kit,EP3 and HCN1 in bladder tissue significantly increased(P<0.05,P<0.01).Compared with the model group,the electroacupuncture group showed a decrease in bladder basal pressure and leak point pressure(P<0.01),while the bladder maximum capacity and compliance increased(P<0.05,P<0.01);the morphology of bladder tissue was relatively normal;the protein and mRNA expressions of c-Kit,EP3 and HCN1 in bladder tissue were all decreased(P<0.05,P<0.01).Compared with the electroacupuncture group,the electroacupuncture+EP3 agonist group showed an increase in bladder basal pressure and leak point pressure(P<0.05,P<0.01),a decrease in bladder maximum capacity and compliance(P<0.05,P<0.01);aggravated pathological damage to bladder tissue;the protein and mRNA expressions of c-Kit,EP3 and HCN1 in bladder tissue increased(P<0.05,P<0.01).Conclusion Electroacupuncture of"Zhongji","Guanyuan"and"Sanyinjiao"alleviates excessive contraction of the bladder detrusor muscle by inhibiting the activation of the EP3-HCN1 pathway,thereby improving urinary incontinence symptoms after suprasacral spinal cord injury in rats.关键词
电针/尿失禁/骶上脊髓损伤/E-前列腺素3受体/超极化激活环核苷酸门控通道蛋白1/大鼠Key words
electroacupuncture/urinary incontinence/suprasacral spinal cord injury/EP3/HCN1/rats分类
医药卫生引用本文复制引用
张静,梁彤,韩润霞,韦玲..基于EP3-HCN1通路探讨电针对骶上脊髓损伤后尿失禁大鼠的效应机制[J].中国中医药信息杂志,2025,32(7):73-80,8.基金项目
山西中医药管理局科研课题计划项目(2023ZYYB2013、2024ZYYB019) （2023ZYYB2013、2024ZYYB019）
