中国中医药信息杂志2025，Vol.32Issue(7)：97-103,7.DOI:10.19879/j.cnki.1005-5304.202501478
化瘀消痞汤调控HIF-1α/VEGF信号通路对AGS细胞增殖、迁移的影响
Effects of Huayu Xiaopi Decoction Regulating HIF-1α/VEGF Signaling Pathway on Proliferation and Migration of AGS Cells
摘要
Abstract
Objective To investigate the effects of Huayu Xiaopi Decoction on the proliferation and migration of AGS cells;To explore its mechanism in treating precancerous lesions of gastric cancer.Methods AGS cells were divided into blank group,inhibitor group and Huayu Xiaopi Decoction high-,medium-and low-dosage groups.The blank group was cultured with 15%control serum,the inhibitor group was cultured with 10 μmol/L HIF-1α inhibitor,and the Huayu Xiaopi Decoction high-,medium-and low-dosage groups were cultured with 12%,6%and 3%drug containing serum,respectively.CCK-8 method was used to detect cell survival rate,cell migration ability was detected by scratch test,immunofluorescence was used to detect the expressions of proliferating cell nuclear antigen(PCNA),matrix metalloproteinase(MMP)-2 and MMP-9 in AGS cells,the expressions of HIF-1α,COX-2,VEGF,VEGFR2,MMP-2,MMP-9 mRNA and HIF-1α,COX-2,VEGF,VEGFR2 proteins in AGS cells were detected by RT-PCR and Western blot.Results Compared with the blank group,the cell survival rate and migration rate were significantly decreased in the inhibitor group and each dosage of Huayu Xiaopi Decoction groups(P<0.05),the expressions of PCNA,MMP-2 and MMP-9 significantly decreased in the inhibitor group and Huayu Xiaopi Decoction high-and medium-dosage groups(P<0.05),the mRNA expressions of HIF-1α,COX-2,VEGF,VEGFR2,MMP-2,MMP-9 and the protein expression of HIF-1α,COX-2,VEGF,VEGFR2 were significantly decreased(P<0.05).Conclusion Huayu Xiaopi Decoction can inhibit the proliferation and migration of AGS cells,and its mechanism is related to the regulation of the expression of key molecules in HIF-1α/VEGF signaling pathway.关键词
胃癌前病变/AGS细胞/化瘀消痞汤/HIF-1α/VEGF信号通路/增殖Key words
precancerous lesions of gastric cancer/AGS cells/Huayu Xiaopi Decoction/HIF-1α/VEGF signaling pathway/proliferation分类
医药卫生引用本文复制引用
郭崇元,魏娜,白敏,公艳霞,李卫强,卢海瑞,安耀荣,段永强..化瘀消痞汤调控HIF-1α/VEGF信号通路对AGS细胞增殖、迁移的影响[J].中国中医药信息杂志,2025,32(7):97-103,7.基金项目
国家中医药管理局重点学科建设项目(2023年) （2023年）
宁夏自然科学基金一般项目(2023AAC03170、2024AAC03741) （2023AAC03170、2024AAC03741）
宁夏医科大学中医学院一流学科建设项目/小荷计划项目(ZY0019110305) （ZY0019110305）
