中国中医药信息杂志2025，Vol.32Issue(7)：104-111,8.DOI:10.19879/j.cnki.1005-5304.202411332
基于Keap1/Nrf2信号通路研究血府逐瘀汤对冠心病心血瘀阻证模型大鼠氧化应激损伤的干预机制
Study on Mechanism of Xuefu Zhuyu Decoction in Interfering Oxidative Stress Injury in Rats with Heart Blood Stasis Syndrome of Coronary Heart Disease Based on Keap1/Nrf2 Signaling Pathway
摘要
Abstract
Objective To investigate the effects and mechanism of Xuefu Zhuyu Decoction in oxidative stress in coronary heart disease model rats with heart blood stasis syndrome based on Keap1/Nrf2 signaling pathway.Methods The rats were divided into normal group,sham-operation group,model group,Xuefu Zhuyu Decoction group and trimetazidine group.The rat model of coronary heart disease with heart blood stasis syndrome was established by ligation of the left anterior descending branch of the coronary artery.Xuefu Zhuyu Decoction group and trimetazidine group were administrated with the corresponding drugs at the dosages of 14.04 g/kg and 5.4 mg/kg,respectively,and normal group,sham-operation group and model group were administrated with the same volume of normal saline for 14 days.The general state of rats was observed,body mass was recorded and electrocardiogram was collected.Echocardiography was used to examine cardiac functions(LVEF,LVFS,LVIDd,LVIDs);the morphology of myocardial tissue was observed by HE staining,serum malondialdehyde(MDA),superoxide dismutase(SOD),glutathione peroxidase(GSH-Px)and total antioxidant capacity(T-AOC)were detected by ELISA,the positive expressions of Keap1,Nrf2,HO-1 and NQO1 in myocardial tissue were detected by immunohistochemistry.Results Compared with the normal group and sham-operation group,the rats in the model group showed signs of mental fatigue,reduced activity,dull fur,purple claws,and a significant decrease in body mass(P<0.01);the ST segment in lead Ⅱ of the electrocardiogram was significantly elevated,LVEF and LVFS were significantly reduced,and LVIDd and LVIDs significantly increased(P<0.01),with severe degeneration and necrosis of myocardial cells,disappearance of striated structures,disordered arrangement of myocardial fibers,infiltration of inflammatory cells;the serum MDA content significantly increased,while the activities of SOD,GSH-Px and T-AOC significantly decreased(P<0.01);the positive expressions of Keap1 and Nrf2 in myocardial tissue significantly increased,while the positive expression of HO-1 and NQO1 significantly decreased(P<0.01).Compared with the model group,the rats in Xuefu Zhuyu Decoction group and trimetazidine group showed improvement in their mental state,increased activity,shiny fur,rosy nails,and significantly increased body mass(P<0.01);the ST segment of the electrocardiogram decreased to varying degrees,with significant increases in LVEF and LVFS,and significant decreases in LVIDd and LVIDs(P<0.01);a large number of myocardial cells survived,the arrangement of myocardial fibers was relatively regular,and the infiltration of inflammatory cells was significantly reduced;the serum MDA content was significantly reduced,while the activities of SOD,GSH-Px and T-AOC significantly increased(P<0.01);the positive expression of Keap1 in myocardial tissue significantly decreased,while the positive expressions of Nrf2,HO-1 and NQO1 significantly increased(P<0.01).Conclusion Xuefu Zhuyu Decoction may inhibit oxidative stress by activating Keap1/Nrf2 signaling pathway to improve the pathological morphology and structural damage of myocardial tissue and promote the recovery of cardiac functions in rats with heart blood stasis syndrome of coronary heart disease.关键词
冠心病/心血瘀阻证/血府逐瘀汤/氧化应激/Keap1/Nrf2信号通路/大鼠Key words
coronary heart disease/heart blood stasis syndrome/Xuefu Zhuyu Decoction/oxidative stress/Keap1/Nrf2 signaling pathway/rats分类
医药卫生引用本文复制引用
匡慧芳,李静,田朋,苏畅,刘祎,王明韵,张秋雁..基于Keap1/Nrf2信号通路研究血府逐瘀汤对冠心病心血瘀阻证模型大鼠氧化应激损伤的干预机制[J].中国中医药信息杂志,2025,32(7):104-111,8.基金项目
国家自然科学基金面上项目(82274411) （82274411）
湖南省自然科学基金部门联合基金(2024JJ8167) （2024JJ8167）
湖南中医药大学学科建设"揭榜挂帅"项目(22JBZ005) （22JBZ005）
湖南省"十四五"第二批中医药高层次人才学科带头人人才培养项目(2024年) （2024年）
湖南中医药大学中药粉体与创新药物省部共建国家重点实验室培育基地开放基金项目(24PTKF1004) （24PTKF1004）
湖南省研究生科研创新项目(CX20230796) （CX20230796）
湖南中医药大学"一方"研究生创新项目(2023YF07) （2023YF07）
