中成药2025，Vol.47Issue(6)：1853-1860,8.DOI:10.3969/j.issn.1001-1528.2025.06.011
没食子酸对三阴乳腺癌HCC1806细胞荷瘤裸鼠移植瘤生长的抑制作用
Inhibitory effect of gallic acid on HCC1806 triple-negative breast cancer xenograft growth in nude mice
摘要
Abstract
AIM To investigate the inhibitory effect of gallic acid on HCC1806 triple-negative breast cancer xenograft growth in nude mice.METHODS The nude mouse models of HCC1806 triple-negative breast cancer xenograft growth were established and randomly divided into the model group,the gallic acid group(200 mg/kg)and the adriamycin group(5 mg/kg),with 6 mice in each group.After 21 days of respective drug administration,the nude mice were killed and had their xenografts procured and measured.The nude mice had their serum levels of IL-6 and TNF-α detected by ELISA;their morphological changes of xenografts observed with HE staining;their protein expressions of p53,PCNA and Ki67 in xenograft detected by immunohistochemistry;their mRNA expressions of PCNA,Ki67,Bcl-2,Bax,Caspase-3,PI3K,Akt,JNK and p38 detected by RT-qPCR;and their protein expressions of Bcl-2,Bax,Caspase-3,cleaved-Caspase-3,PI3K,p-PI3K,Akt,p-Akt,JNK,p-JNK,p38 MAPK and p-p38 MAPK in xenografts detected by Western blot.RESULTS Compared with the model group,the groups intervened with either gallic acid or adriamycin shared decreased tumor weight(P＜0.05);increased serum levels of IL-6 and TNF-α(P＜0.05,P＜0.01);condensed or irregular nuclei of xenograft,and increased number of cell necrosis;increased protein expressions of p53 and Bax and the ratios of cleaved-Caspase-3/Caspase-3,p-JNK/JNK and p-p38/p38 in xenograft(P＜0.01);decreased protein expressions of PCNA,Ki67 and Bcl-2 and the ratios of p-PI3K/PI3K and p-Akt/Akt(P＜0.01);decreased mRNA expressions of PCNA,Ki67,Bcl-2,PI3K and Akt(P＜0.05,P＜0.01);and increased mRNA expressions of Caspase-3,Bax,JNK and p38(P＜0.05,P＜0.01).CONCLUSION Gallic acid can inhibit HCC1806 triple-negative breast cancer xenograft growth in nude mice,and the underlying mechanism may involve suppression of pro-cell proliferation proteins,activation of pro-apoptotic proteins,and modulation of the PI3K/Akt and JNK/p38 signaling pathways.关键词
没食子酸/三阴乳腺癌/HCC1806细胞/增殖/凋亡/PI3K/Akt信号通路/JNK/p38信号通路Key words
gallic acid/triple-negative breast cancer/HCC1806 cells/proliferation/apoptosis/PI3K/Akt signaling pathway/JNK/p38 signaling pathway分类
医药卫生引用本文复制引用
江春娟,林思,秦慧真,黄捷..没食子酸对三阴乳腺癌HCC1806细胞荷瘤裸鼠移植瘤生长的抑制作用[J].中成药,2025,47(6):1853-1860,8.基金项目
广西科技基地和人才专项(桂科AD21238031) （桂科AD21238031）
广西重点研发项目(桂科AB21196016) （桂科AB21196016）
广西壮瑶药协同创新中心项目(桂教科研[2013]20号) （桂教科研[2013]20号）
