中医临床研究2025，Vol.17Issue(8)：1-8,8.DOI:10.3969/j.issn.1674-7860.2025.08.001
基于网络药理学探讨山慈菇-夏枯草治疗乳腺增生的潜在作用机制
Exploring the potential action mechanism of Shancigu-Xiakucao in the treatment of breast hyperplasia based on network pharmacology
摘要
Abstract
Objective:Network pharmacology research method was used to analyze the potential targets and action mechanisms of Shancigu(Pseudobulbus Cremastrae seu Pleiones)-Xiakucao(Prunellae Spica)in the the treatment of breast hyperplasia.Methods:The Traditional Chinese Medicine System Pharmacology Database and Analysis Platform(TCMSP)was employed to retrieve the active constituents of Shancigu-Xiakucao and their respective targets.The GeneCards and OMIM databases were utilized to obtain the targets associated with breast hyperplasia.The intersection of potential targets of the medicines and disease was taken to drawn a Venny diagram that visually represented the common targets.Cytoscape 3.9.2 software and STRING database were employed to construct the active ingredients-targets network diagram and protein-protein interaction network diagram,respectively.Furthermore,R software was utilized to perform Gene Ontology(GO)enrichment analysis and Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway enrichment analysis on the intersecting genes.The AutoDock 1.5.6 software was used for molecular docking,and PyMOL 3.0.3 software was used for visual analysis.Results:The study successfully identified 13 active ingredients of Chinese medicines,193 corresponding targets,5 088 disease targets associated with breast hyperplasia,and 162 medicine-disease common targets.A total of 44 core targets were obtained from the PPI network analysis.The main active ingredients included quercetin,β-sitosterol,stigmasterol,luteolin,and 2-methoxy-9,10-dihydrophenanthrene-4,5-diol,etc.,which acted on core targets such as AKT1,TNF,IL-6,and TP53 by regulating AGE-RAGE signaling pathway,TNF signaling pathway,as well as IL-17 signaling pathway to enhance cellular resistance to oxidative stress,regulate hormonal levels,inhibit angiogenesis,and inhibit inflammatory,thereby treating breast hyperplasia.The molecular docking results showed that the main active components had good binding ability with the targets.Conclusion:Shancigu-Xiakucao could treat breast hyperplasia through multiple components,targets and pathways,providing a theoretical basis and reference for clinical treatment and experimental exploration.关键词
网络药理学/山慈菇-夏枯草/乳腺增生Key words
Network pharmacology/Shancigu-Xiakucao/Breast hyperplasia分类
医药卫生引用本文复制引用
张名明,樊舒瑶,谢小红..基于网络药理学探讨山慈菇-夏枯草治疗乳腺增生的潜在作用机制[J].中医临床研究,2025,17(8):1-8,8.基金项目
浙江省中医药科技计划项目(2024ZL379). （2024ZL379）
