中国药理学通报2025，Vol.41Issue(7)：1237-1244,8.DOI:10.12360/CPB202410044
两色金鸡菊总黄酮抑制miR-93调控TLR4信号通路改善血管性痴呆的作用及机制
Effect of total flavones of Coreopsis tinctoria Nutt on vascular dementia by inhibiting miR-93-mediated TLR4 signaling pathway and its mechanism
摘要
Abstract
Aim To investigate the effect of total fla-vones of Coreopsis tinctoria Nutt(CF)on cognitive im-pairment in vascular dementia(VD).Methods The VD rat models were established by modified bilateral common carotid arteries ligation method.SD rats were divided into the sham operation group,model group,positive control group(nicergoline),and low,medium,and high dose CF groups.After eight weeks of admin-istration,the short term memory and spatial learning and memory abilities were evaluated by the platform jumping test,dark avoidance test and Morris water maze test.The pathological changes of the hippocam-pal tissues were inspected by HE and Nissl staining.The contents of TNF-α and IL-1β in the hippocampal were examined by ELISA.The protein expression lev-els of TLR4,MyD88,NF-κB p65,and p-NF-κB p65 in the hippocampal were detected by Western blot.The mRNA expression levels of miR-93,TLR4,MyD88,and NF-κB p65 in the hippocampal were determined by qRT-PCR.Results CF obviously improved the short term memory and spatial learning and memory abilities of VD rats,and alleviated the pathological damage of the hippocampus.CF also obviously decreased the lev-els of TNF-α and IL-1β,declined the protein expres-sion levels of TLR4,MyD88,and p-NF-κB p65,and re-duced the miR-93,TLR4,and MyD88 mRNA expres-sion in the hippocampus.Conclusion CF has a nota-ble protective effect on the neuroinflammation and cog-nitive impairments in VD rats by inhibiting the miR-93-mediated TLR4 signaling pathway.关键词
两色金鸡菊总黄酮/血管性痴呆/miR-93/TLR4通路/认知障碍/炎症反应Key words
total flavones of Coreopsis tinctoria Nutt/vascular dementia/miR-93/TLR4 signaling pathway/cognitive impairment/inflammatory response分类
医药卫生引用本文复制引用
胡梦颖,杨冬梅,朱毅忠,梁钦兰,努尔巴合提·候瓦提,杨晓君,哈木拉提·哈斯木..两色金鸡菊总黄酮抑制miR-93调控TLR4信号通路改善血管性痴呆的作用及机制[J].中国药理学通报,2025,41(7):1237-1244,8.基金项目
国家自然科学基金资助项目(No 82060789) （No 82060789）
新疆维吾尔自治区自然科学基金面上项目(No 2022D01A133) （No 2022D01A133）
