中国药理学通报2025，Vol.41Issue(7)：1265-1273,9.DOI:10.12360/CPB202410094
灵孢多糖促进双环己酮草酰二腙诱导的脱髓鞘小鼠髓鞘再生的机制
Mechanism of Ganoderma lucidum polysaccharides promoting myelin regeneration in demyelinated mice induced by cuprizone
摘要
Abstract
Aim To explore the mechanism of Gano-derma lucidum polysaccharides(GLPS)promoting my-elin repair and regeneration in mice with chronic demy-elination induced by cuprizone(CPZ).Methods A total of 40 C57BL/6 mice were randomly divided into four groups:Normal+NS,Normal+GLPS,CPZ+NS and CPZ+GLPS.A chronic demyelination model was established using 0.2％CPZ.Open field and elevated plus maze tests were performed to observe the behavior-al changes in the mice.Immunofluorescence staining and Western blot were used to detect changes in myelin basic protein expression in the corpus callosum.ELISA was performed to measure the levels of TNF-α,IL-6,IL-1β and IL-10 in brain homogenates.Immunofluo-rescence staining was also used to observe the expres-sion of ionized calcium binding adapter molecule 1(Iba1)and neural-glial antigen 2(NG2).RT-qPCR and Western blot were conducted to assess the mRNA and protein expression levels of MBP,iNOS,COX-2,JAK2 and STAT3.Results Mice in the CPZ+NS group showed a significant decrease in body weight,cognitive behavior abnormalities,and impaired myelin regeneration.The expression of pro-inflammatory fac-tors increased,while anti-inflammatory factors de-creased.Additionally,Iba1 and NG2 expression in-creased,and the JAK2/STAT3 signaling pathway was activated.After GLPS intervention,the mouse body weight increased,myelin regeneration occurred,cogni-tive behavior was improved,the expression of inflamma-tory factors decreased,anti-inflammatory factors in-creased,NG2 expression was further elevated,and the proliferation of microglia as well as the activation of the JAK2/STAT3 signaling pathway was inhibited.Conclu-sions GLPS can improve cognitive behavior abnormali-ties and inflammatory responses in chronic demyelinated mice by inhibiting the JAK2/STAT3 signaling pathway,thereby promoting myelin repair and regeneration.关键词
灵孢多糖/双环己酮草酰二腙/多发性硬化/髓鞘修复与再生/炎症/JAK2/STAT3信号通路Key words
Ganoderma lucidum polysaccharides/cu-prizone/multiple sclerosis/myelin repair and regener-ation/inflammation/JAK2/STAT3 signaling pathway分类
医药卫生引用本文复制引用
李彦青,李晓慧,王青,宋丽娟,杨立志,王翰斌,肖保国,马存根..灵孢多糖促进双环己酮草酰二腙诱导的脱髓鞘小鼠髓鞘再生的机制[J].中国药理学通报,2025,41(7):1265-1273,9.基金项目
国家自然科学基金资助项目(No 82304752) （No 82304752）
山西省横向课题(No 2022HX-GR-01) （No 2022HX-GR-01）
山西省卫生健康科研课题(No 2022127) （No 2022127）
山西省卫健委医学科技领军团队(No 2020TD05) （No 2020TD05）
