中国药理学通报2025，Vol.41Issue(7)：1290-1297,8.DOI:10.12360/CPB202410099
黄芪甲苷通过cGAS/STING/NF-κB通路抑制LPS诱导的RAW 264.7巨噬细胞极化调控其迁移
Astragaloside Ⅳ inhibits LPS-induced RAW 264.7 macrophage polarization and regulates their migration via cGAS/STING/NF-κB pathway
摘要
Abstract
Aim To explore the effect of astragalosideⅣ(AS-Ⅳ)on lipopolysaccharide(LPS)-induced po-larization and migration of RAW 264.7 macrophages and the underlying mechanism.Methods 1 mg·L-1 LPS was used to construct cell migration model.Scratch assay was utilized to determine cell migration rate.Immunofluorescence staining was utilized to de-tect the expression and location of F4/80,iNOS and Arg-1.CCK-8 assay was used to determine the viabili-ty of RAW 264.7 cells.Griess assay was used to measure NO content.Molecular docking was used to analyze the interaction between AS-Ⅳ and the core tar-gets such as cGAS and STING protein.Western blot was employed to detect the expression of iNOS,Arg-1,cGAS,STING,NF-κB p65 and p-NF-κB p65 protein.Results AS-Ⅳ significantly inhibited the migration and M1 polarization of RAW 264.7 cells induced by LPS.Moreover,AS-Ⅳ could interact with cGAS and STING protein,especially cGAS.Further Western blot assay showed that AS-Ⅳ significantly downregulated the expression of iNOS,cGAS,STING and p-NF-κB p65 protein.Conclusions AS-Ⅳ could promote mac-rophage M1 to M2 polarization,thereby inhibited mac-rophage migration through restraining the cGAS/STING/NF-κB signaling pathway,which provides a new therapeutic target for AS-Ⅳ to improve the early inflammatory response of AS.关键词
黄芪甲苷/巨噬细胞极化/巨噬细胞迁移/分子对接/脂多糖/cGAS/STING/NF-κBKey words
astragaloside Ⅳ/macrophage polariza-tion/macrophage migration/molecular docking/li-popolysaccharide/cGAS/STING/NF-κB分类
医药卫生引用本文复制引用
杨长超,李国婷,刘琳,赵梓先,李伟慷,孙庆新,赵钰莹,赵京山..黄芪甲苷通过cGAS/STING/NF-κB通路抑制LPS诱导的RAW 264.7巨噬细胞极化调控其迁移[J].中国药理学通报,2025,41(7):1290-1297,8.基金项目
河北省重点研发计划项目中医药创新专项(No 223777149D) （No 223777149D）
河北省自然科学基金项目(No H2020423061,H2023423028) （No H2020423061,H2023423028）
