中国药理学通报2025，Vol.41Issue(7)：1359-1367,9.DOI:10.12360/CPB202409015
异槲皮苷通过调控海马脑区BDKRB2/PI3K/Akt信号通路对焦虑症大鼠的改善作用
Improvement effect of isoquercitrin on anxiety rats through modulation of BDKRB2/PI3K/Akt pathway in hippocampus
摘要
Abstract
Aim To explore the action mechanism of isoquercitrin(IQ)in ameliorating anxiety based on network pharmacology,cellular transcriptomics,molecu-lar docking and animal experiments.Methods The common targets of anxiety disorders and IQ were ob-tained by using relevant databases.The protein-protein interaction network,the biological function and signa-ling pathway enrichment analysis were conducted by u-sing the common targets.Primary hippocampal neurons were cultured in vitro,and corticosterone was added to induce neurons to establish a corticosterone injury mod-el.IQ treatment was added to the culture system,and transcriptomics was used to screen for differentially ex-pressed genes and enrich for differentially expressed pathways.Subsequently,the results were validated by quantitative real-time polymerase chain reaction(qRT-PCR).Possible targets and signaling pathways for IQ treatment on anxiety were speculated and screened u-sing network pharmacology,transcriptomics and molec-ular docking.The anxiety rat model was constructed,and the anxiety state of rats was evaluated after IQ in-tervention,and the protein expression level of hippo-campus was detected to verify the relevant mechanism.Results Network pharmacology,cellular transcrip-tome,and molecular docking analyses revealed that the key mechanism of IQ for anxiety may be related to the BDKRB2/PI3K/Akt signaling pathway.Animal exper-iments showed that IQ was effective in improving anxie-ty behaviour and learning memory ability in rats.IQ increased the movement distance and residence time in the central area of the open field,the time and number percentage of entries into the open arm in the elevated plus maze,and the spontaneous alternations score in the Y maze in rats,and significantly elevated protein expression of BDKRB2,PI3K,Akt and decreased pro-tein expression of NF-κB in the hippocampus.Conclu-sions Isoquercitrin can effectively treat anxiety,and the mechanism of action may be related to the regula-tion of BDKRB2/PI3K/Akt signaling pathway in hip-pocampus.关键词
异槲皮苷/焦虑症/海马/网络药理学/转录组学/作用机制Key words
isoquercitrin/anxiety/hippocampus/net-work pharmacology/transcriptomics/action mechanism引用本文复制引用
郭德第,张艺伟,王香君,陈玺泰,赵怀伟,宋润蔚,王常瞵,宋春红..异槲皮苷通过调控海马脑区BDKRB2/PI3K/Akt信号通路对焦虑症大鼠的改善作用[J].中国药理学通报,2025,41(7):1359-1367,9.基金项目
国家重大新药创制项目(No 2017ZX09301064) （No 2017ZX09301064）
国家自然科学基金青年资助项目(No 81403294) （No 81403294）
山东省自然科学基金面上资助项目(No ZR2020MH341) （No ZR2020MH341）
山东省中医药科技项目(No 2021M121) （No 2021M121）
济南市中心医院常青树项目(No CQS2020009) （No CQS2020009）
