中国现代中药2025，Vol.27Issue(6)：1018-1030,13.DOI:10.13313/j.issn.1673-4890.20250206002
基于UHPLC-Q-Exactive Orbitrap MS的野棉花地上部分化学成分分析
Analysis of Chemical Constituents in Aerial Parts of Anemone vitifolia Based on UHPLC-Q-Exactive Orbitrap MS
周道堂 1段慧 1瓦庆毕 1吴莹莹 2彭潇 3孙绪4
作者信息
- 1. 凯里市第一人民医院 药剂科,贵州 凯里 556000
- 2. 毕节医学高等专科学校 药学系,贵州 毕节 551602
- 3. 浙江省人民医院毕节医院/毕节市第一人民医院 药学部,贵州 毕节 551700
- 4. 贵州省食品药品检验所,贵州 贵阳 550081
- 折叠
摘要
Abstract
Objective:Ultra-performance liquid chromatography-tandem Q-Exactive quadrupole-electrostatic field track trap high resolution mass spectrometry(UHPLC-Q-Exactive Orbitrap MS)was used to rapidly identify the chemical constituents in the aerial parts of Anemone vitifolia.Methods:A Waters Acquity UPLC BEH C18 column(150 mm×2.1 mm,1.7 μm)was used with a gradient elution employing 0.1%formic acid solution(A)and acetonitrile(B)as mobile phase.The flow rate was 0.3 mL·min-1;the column temperature was 40℃,and the injection volume was 1 μL.The electrospray ionization(ESI)source was adopted for the analysis in positive and negative ion modes.The precise relative molecular mass and characteristic fragment ions of the compounds in the aerial parts of A.vitifolia samples were compared with the reference substances or the compounds reported in the literature.Results:A total of 115 compounds were identified from the aerial parts of A.vitifolia,including 32 organic acids and organic acid esters,21 flavonoids,19 alkaloids,13 phenylpropanoids,5 amino acids,5 steroids,4 nucleosides,4 saccharides,4 terpenoids,and 8 other constituents.Conclusion:The UHPLC-Q-Exactive Orbitrap MS method can rapidly analyze and identify the chemical constituents in the aerial parts of A.vitifolia,providing a reference for further research on the material basis of its efficacy,mechanism of action,and product development.关键词
超高效液相色谱-四极杆-静电场轨道阱高分辨质谱/野棉花/化学成分Key words
UHPLC-Q-Exactive Orbitrap MS/Anemone vitifolia Buch.-Ham./chemical constituents分类
医药卫生引用本文复制引用
周道堂,段慧,瓦庆毕,吴莹莹,彭潇,孙绪..基于UHPLC-Q-Exactive Orbitrap MS的野棉花地上部分化学成分分析[J].中国现代中药,2025,27(6):1018-1030,13.
