中国现代中药2025，Vol.27Issue(6)：1031-1039,9.DOI:10.13313/j.issn.1673-4890.20240812001
三七须根指纹图谱建立及皂苷类成分含量测定
Fingerprint of Panax notoginseng Fibrous Root and Content Determination of Its Ginsenosides
摘要
Abstract
Objective:To establish the high-performance liquid chromatography(HPLC)fingerprint of Panax notoginseng fibrous root,compare the differences in major ginsenosides between the fibrous root and main root,and analyze the influence of multiple factors on the content of notoginsenoside R2(S)and ginsenoside Rh1 in the fibrous root.Methods:HPLC was used to establish the fingerprint,and the Traditional Chinese Medicine Chromatographic Fingerprint Similarity Evaluation System(version 2.0)was applied to evaluate similarity and identify common peaks.Additionally,the content of major ginsenosides in the fibrous root and main root was determined.Results:The HPLC fingerprint of P.notoginseng fibrous root was established,with seven identified components:notoginsenoside R1,ginsenoside Rg1,ginsenoside Re,notoginsenoside R2(S),ginsenoside Rb1,ginsenoside Rh1,and ginsenoside Rd.The proportions of notoginsenoside R2(S)and ginsenoside Rh1 in total saponins were significantly higher in the fibrous root than in the main root.Among different drying methods,the content of notoginsenoside R2(S)and ginsenoside Rh1 followed the order:fresh sample>oven-dried sample>shade-dried sample>sun-dried sample.Conclusion:The established HPLC fingerprint of P.notoginseng fibrous root is stable and reliable.A comprehensive analysis of the content of notoginsenoside R2(S)and ginsenoside Rh1 provides a reference for the development and utilization of P.notoginseng fibrous root.关键词
三七须根/指纹图谱/三七皂苷R2(S)/人参皂苷Rh1Key words
Panax notoginseng(Burk)F.H.Chen fibrous root/fingerprint/notoginsenoside R2(S)/ginsenoside Rh1分类
中医学引用本文复制引用
张红燕,杨淳皓,杨兆祥,肖丰坤..三七须根指纹图谱建立及皂苷类成分含量测定[J].中国现代中药,2025,27(6):1031-1039,9.基金项目
云南特色植物提取实验室自主研究项目(2022YKZY004,2023YKZY003) （2022YKZY004,2023YKZY003）
