中国现代中药2025，Vol.27Issue(6)：1049-1058,10.DOI:10.13313/j.issn.1673-4890.20240606004
基于HPLC指纹图谱和网络药理学探究连翘保肝作用潜在机制
Potential Mechanisms of Forsythia suspensa Against Liver Injury Based on HPLC Fingerprint and Network Pharmacology
摘要
Abstract
Objective:To investigate the pharmacologically active substances and mechanisms underlying the hepatoprotective effects of Forsythia suspensa using HPLC fingerprinting and network pharmacology.Methods:HPLC fingerprints were established for 28 batches of F.suspensa,and characteristic peaks were identified using reference materials.Further evaluation was conducted by combining similarity analysis,cluster analysis,and partial least squares discriminant analysis(PLS-DA)to screen for pharmacologically relevant substances,followed by network pharmacology analysis.Component-related targets were retrieved from the SwissTargetPrediction database,while liver injury-related targets were obtained from the OMIM,GeneCards,and DrugBank databases.Intersection targets between the components and the disease were selected,and a protein-protein interaction(PPI)network was constructed using the STRING database,followed by topological parameter analysis.Cytoscape 3.8.2 was used to construct a"component-disease-target"network diagram,and Gene Ontology(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG)enrichment analyses were performed on the intersection targets using the Metascape website.Results:The similarity of the HPLC fingerprints of F.suspensa was greater than 0.900.Through cluster analysis,the 28 batches of samples were grouped into three categories.The results of PLS-DA were consistent with those of the cluster analysis.Based on comparison with reference materials and comprehensive analysis,forsythoside E,pinoresinol diglucoside,forsythoside A,isoquercitrin,and phillyrin were identified as pharmacologically relevant substances.These substances may exert their effects by regulating core targets such as HRAS,PTGS2,MCL1,IL2,and HSP90AA1,as well as key signaling pathways including the IL-17 signaling pathway,arachidonic acid metabolism signaling pathway,and PI3K-Akt signaling pathway.Conclusion:By combining HPLC fingerprint and network pharmacology,this study explored the pharmacologically active substances and mechanisms associated with the hepatoprotective effects of F.suspensa,providing a reference for quality control,variety selection,and clinical research on F.suspensa.关键词
连翘/高效液相色谱法/指纹图谱/网络药理学/肝损伤保护Key words
Forsythia suspensa(Thunb.)Vahl/HPLC/fingerprint/network pharmacology/liver protection分类
医药卫生引用本文复制引用
田溪,李雯钰,张惠怡,李红艳,刘灵娣,温春秀..基于HPLC指纹图谱和网络药理学探究连翘保肝作用潜在机制[J].中国现代中药,2025,27(6):1049-1058,10.基金项目
财政部和农业农村部:国家现代农业产业技术体系资助项目 （）
河北省农林科学院科技创新人才队伍建设项目(C23R0804) （C23R0804）
