中国现代中药2025，Vol.27Issue(6)：1059-1071,前插1-前插2,15.DOI:10.13313/j.issn.1673-4890.20250312007
枸杞红瘿蚊成虫触角转录组及嗅觉相关基因分析
Antennal Transcriptome and Olfaction-related Genes of Gephyraulus lycantha(Diptera:Cecidomyiidae)
摘要
Abstract
Objective:To obtain the antennal transcriptome data of Gephyraulus lycantha Jiao&Kolesik and mine the olfaction-related genes.Methods:The Illumina NovaSeq 6000 platform was used for transcriptome sequencing of the antennae of male and female adults of G.lycantha,and gene annotation was performed across multiple public databases,including Non-Redundant Protein Database(NR),Nucleotide Sequence Database(NT),Protein Families Database(Pfam),Non-Redundant Protein Database(KOG)/Clusters of Orthologous Groups of proteins(COG),Swiss-Prot,Kyoto Encyclopedia of Genes and Genomes(KEGG)Orthologue(KO),and Gene Ontology(GO).Bioinformatics and phylogenetic analyses were then carried out for the olfaction-related genes screened out.The differentially expressed genes in male and female antennae were validated by using quantitative real-time PCR(qRT-PCR).Results:A total of 54 105 unigenes were obtained,with an average length of 1 102 bp and a N50 of 1 841 bp.Among them,19 013 unigenes were successfully annotated in at least one database.Functional annotation identified 152 olfaction-related genes,including 27 odorant-binding proteins(OBPs),3 chemosensory proteins(CSPs),86 odorant receptors(ORs),15 gustatory receptors(GRs),15 ionotropic receptors(IRs),and 6 sensory neuron membrane proteins(SNMPs).Phylogenetic analysis revealed three GlycOBPs clustered in the OS-E/OS-F clade.GlycCSPs exhibited high bootstrap support with other dipteran homologs.Sixteen GlycORs(GlycOR3/7/9/11/14/23/26/44/53/60/61/67/71/80/83/85)formed a distinct species-specific clade.Both GlycIRs and GlycGRs showed strong phylogenetic congruence with orthologs from other dipteran species.Notably,GlycSNMP1 and GlycSNMP2 were clustered into the conserved SNMP1 and SNMP2 clades,respectively,with high bootstrap support among dipteran species.The qRT-PCR results demonstrated six differentially expressed genes(encoding GlycOBP2,GlycOBP14,GlycOBP15,GlycOR3,GlycOR9,and GlycOR85)related to olfaction in male and female antennae,which were consistent with transcriptome sequencing results.Conclusion:The results significantly enrich the available information on the olfaction-related genes of G.lycantha and lay a molecular foundation for delving into the olfactory recognition mechanisms and controlling this pest by targeting olfaction-related genes.关键词
枸杞红瘿蚊/触角转录组/基因注释/嗅觉相关基因Key words
Gephyraulus lycantha Jiao&Kolesik/antennal transcriptome/gene annotation/olfaction-related genes分类
中医学引用本文复制引用
陈丽娟,张雨晴,魏红爽,徐常青,刘赛,乔海莉..枸杞红瘿蚊成虫触角转录组及嗅觉相关基因分析[J].中国现代中药,2025,27(6):1059-1071,前插1-前插2,15.基金项目
国家重点研发计划项目(2022YFC3501502) （2022YFC3501502）
中央本级重大增减支项目(2060302) （2060302）
中国医学科学院医学与健康科技创新工程项目(2021-I2M-1-031) （2021-I2M-1-031）
