中国现代中药2025，Vol.27Issue(6)：1072-1080,9.DOI:10.13313/j.issn.1673-4890.20250106006
外源辅酶Q10对新疆紫草毛状根紫草素类成分含量和抗氧化酶活性的影响
Effect of Exogenous Coenzyme Q10 on Shikonin Compound Content and Antioxidant Activity in Hairy Roots of Arnebia euchroma
摘要
Abstract
Objective:This study aims to investigate the effects of exogenous coenzyme Q10 on the growth of hairy roots of Arnebia euchroma,the content of total naphthoquinone and shikonin compounds,and the activity of antioxidant enzymes,thereby providing a reference for reducing the browning of the hairy roots and increasing shikonin content.Methods:Exogenous coenzyme Q10 at a concentration of 20 μmol·L-1 was added during the cultivation process of hairy roots of A.euchroma.The biomass,total naphthoquinone and shikonin compound contents,antioxidant enzyme activities,reactive oxygen species(ROS)levels,and malondialdehyde(MDA)content of hairy roots of A.euchroma were measured.In addition,transcriptome sequencing analysis was performed on the hairy root samples.Results:Compared with the control group,coenzyme Q10 treatment significantly increased the content of total naphthoquinone and shikonin compounds(P<0.05).Moreover,the activities of superoxide dismutase,catalase,and peroxidase were significantly enhanced(P<0.01),while ROS and MDA levels were significantly reduced(P<0.01).Transcriptome analysis identified a total of 561 differentially expressed genes,of which 154 were upregulated,and 407 were downregulated.Gene function enrichment analysis showed that these genes were mainly involved in defense responses,antioxidant responses,and redox pathways related to monooxygenase activity.Furthermore,the expression levels of antioxidant enzyme genes related to ROS scavenging were significantly upregulated.Conclusion:Exogenous coenzyme Q10 can regulate the antioxidant enzyme activity and related metabolic pathways in A.euchroma hairy roots,,significantly increase the content of shikonin compounds and effectively mitigate the browning of hairy roots.关键词
新疆紫草/辅酶Q10/紫草素/抗氧化活性/毛状根Key words
Arnebia euchroma(Royle)Johnst./coenzyme Q10/shikonin/antioxidant activity/hairy root分类
医药卫生引用本文复制引用
李齐,刘长征,罗文杰,宋俊,刘芸芸,王瑞杉,张成才,王升..外源辅酶Q10对新疆紫草毛状根紫草素类成分含量和抗氧化酶活性的影响[J].中国现代中药,2025,27(6):1072-1080,9.基金项目
国家自然科学基金项目(82173934) （82173934）
中国中医科学院科技创新工程项目(CI2021B013,CI2024C001YN,CI2023E002) （CI2021B013,CI2024C001YN,CI2023E002）
财政部和农业农村部:国家现代农业产业技术体系资助项目 （）
江西省科技创新基地计划-引进共建研发机构项目(20222CCH45004) （20222CCH45004）
