中国现代中药2025，Vol.27Issue(6)：1183-1189,7.DOI:10.13313/j.issn.1673-4890.20241014002
分子鉴定技术在中成药组方药味鉴定中的应用研究进展
Research Progress on the Application of Molecular Identification Technology in the Identification of Ingredients in Chinese Patent Medicine Formulations
摘要
Abstract
With the continuous expansion of Chinese patent medicine(CPM)market,ensuring its quality and safety has become a key concern.Traditional identification methods have certain limitations when dealing with complex samples.In recent years,molecular biology techniques have been widely applied to the identification of biological components in CPM.DNA barcoding technology has made significant progress in the identification of single-component CPM.Novel polymerase chain reaction(PCR technologies),such as real-time quantitative PCR(qRT-PCR)and droplet digital PCR(ddPCR),have improved detection sensitivity and accuracy.The development of mini-barcode technology has enhanced the success rate of identifying degraded samples and enabled species-specific recognition.DNA chip technology enables efficient and precise identification of the genetic characteristics of Chinese medicinal materials.For complex samples,amplicon metabarcoding(AMB)technology provides a more accurate method for species detection,though it remains susceptible to PCR bias.Shotgun metagenomic sequencing,which does not require PCR amplification,demonstrates potential for species identification in complex samples.Additionally,single-molecule real-time(SMRT)sequencing,a third-generation sequencing technology,offers broader prospects for CPM identification.This study summarizes recent advancements in molecular identification methods for CPM and proposes that future research should focus on addressing technical bottlenecks such as DNA extraction,data analysis,and database development.It emphasizes the need to advance standardized and intelligent tools to provide more reliable technical support for CPM quality control and market regulation.关键词
中成药/分子鉴定/DNA条形码/宏条形码/微条形码/DNA芯片/单分子实时测序Key words
Chinese patent medicine/molecular identification/DNA barcoding/metabarcoding/mini-barcode/DNA chip/single-molecule real-time分类
医药卫生引用本文复制引用
周士琳,张艳梅,周婷,杨秉倩,牛俊梅,刘振稳,周静..分子鉴定技术在中成药组方药味鉴定中的应用研究进展[J].中国现代中药,2025,27(6):1183-1189,7.基金项目
云南省科技厅科技计划项目(202201AT070118) （202201AT070118）
云南省万人计划"青年拔尖人才"项目(YNWRQNBJ-2019-208) （YNWRQNBJ-2019-208）
