中国中西医结合杂志2025，Vol.45Issue(6)：722-727,6.DOI:10.7661/j.cjim.20241213.159
姜黄素激活PXR上调PDCD4抑制HepG2细胞增殖和迁移
Curcumin Inhibits Proliferation and Migration in HepG2 Cells by Upexpression of PDCD4 and PXR
摘要
Abstract
Objective To investigate the effect of curcumin on the proliferation and migration in human hepatoma HepG2 cells,and explore the underlying molecular mechanism.Methods HepG2 cells were cultured in vitro and treated with different concentrations of curcumin(0,2.5,5,10,20,50 μmol/L)for 48 hours.The cell viability was detected by the cell morphology observation and cell counting kit-8(CCK-8)methods.The cell migration was observed by the cell scratch assay.HepG2 cells were pretreated with sulforaphane[SFN,a common suppressor of pregnane X receptor(PXR)]for 4 hours and then stimulated with curcumin.The mRNA and protein expressions of programmed cell death factor 4(PDCD4),PXR and target genes including multidrug resistance gene 1(MDR1)and cytochrome P450 3A4(CYP3A4)were detected by the qRT-PCR and Western Blot respectively.Results Compared with control group,the survival rate of HepG2 cells in 5,10,20 and,50 μmol/L curcumin groups decreased(P＜0.001,P＜0.01,P＜0.05),the cell migration rate of the 2.5,5,10 μmol/L groups decreased after 72 hours(P＜0.01,P＜0.05).Compared with control group,the protein and mRNA expression of PDCD4 increased in the 20 and 50 μmol/L groups(P＜0.01,P＜0.05),PXR and its target genes MDR1 and CYP3A4 also significantly increased(P＜0.001,P＜0.01,P＜0.05),SFN administration alleviated PDCD4 expression induced by curcumin(P＜0.001).Conclusion Curcumin can inhibit the HepG2 cells proliferation and migration,and its mechanism might be related to upregulation of PDCD4 by activating PXR.关键词
姜黄素/HepG2细胞/孕烷X受体/程序性细胞死亡因子4/细胞增殖迁移/中药提取物/中西医结合Key words
curcumin/HepG2 cells/pregnane X receptor/programmed cell death factor 4/cell proliferation and migration/Chinese herbal extracts/integrative medicine引用本文复制引用
吕依晨,杜旭江,吴培轩,王卓颜,王少兰..姜黄素激活PXR上调PDCD4抑制HepG2细胞增殖和迁移[J].中国中西医结合杂志,2025,45(6):722-727,6.基金项目
国家自然科学基金资助项目(No.81904038) （No.81904038）
陕西省教育厅重点实验室项目(No.23JS011) （No.23JS011）
陕西中医药大学校级课题(No.2023GP12) （No.2023GP12）
陕西省自然科学面上项目(No.2024JC-YBMS-669) （No.2024JC-YBMS-669）
陕西省大学生创新创业项目(No.S202310716034) （No.S202310716034）
