mLife2025，Vol.4Issue(2)：P.169-180,12.DOI:10.1002/mlf2.70007
A CRISPR-nonhomologous end-joining-based strategy for rapid and efficient gene disruption in Mycobacterium abscessus
Sanshan Zeng 1Yanan Ju 2Md Shah Alam 1Ziwen Lu 1H.M.Adnan Hameed 3Lijie Li 2Xirong Tian 1Cuiting Fang 4Xiange Fang 1Jie Ding 5Xinyue Wang 6Jinxing Hu 7Shuai Wang 8Tianyu Zhang9
作者信息
- 1. State Key Laboratory of Respiratory Disease,Guangzhou Institutes of Biomedicine and Health,Chinese Academy of Sciences,Guangzhou,China Guangdong‐Hong Kong‐Macao Joint Laboratory of Respiratory Infectious Diseases,Guangzhou Institutes of Biomedicine and Health,Chinese Academy of Sciences,Guangzhou,China China‐New Zealand Joint Laboratory on Biomedicine and Health,Guangzhou Institutes of Biomedicine and Health,Chinese Academy of Sciences,Guangzhou,China University of Chinese Academy of Sciences,Beijing,China
- 2. State Key Laboratory of Respiratory Disease,Guangzhou Institutes of Biomedicine and Health,Chinese Academy of Sciences,Guangzhou,China Guangdong‐Hong Kong‐Macao Joint Laboratory of Respiratory Infectious Diseases,Guangzhou Institutes of Biomedicine and Health,Chinese Academy of Sciences,Guangzhou,China China‐New Zealand Joint Laboratory on Biomedicine and Health,Guangzhou Institutes of Biomedicine and Health,Chinese Academy of Sciences,Guangzhou,China School of Basic Medical Sciences,Division of Life Science and Medicine,University of Science and Technology of China,Hefei,China
- 3. State Key Laboratory of Respiratory Disease,Guangzhou Institutes of Biomedicine and Health,Chinese Academy of Sciences,Guangzhou,China Guangdong‐Hong Kong‐Macao Joint Laboratory of Respiratory Infectious Diseases,Guangzhou Institutes of Biomedicine and Health,Chinese Academy of Sciences,Guangzhou,China China‐New Zealand Joint Laboratory on Biomedicine and Health,Guangzhou Institutes of Biomedicine and Health,Chinese Academy of Sciences,Guangzhou,China
- 4. State Key Laboratory of Respiratory Disease,Guangzhou Institutes of Biomedicine and Health,Chinese Academy of Sciences,Guangzhou,China Guangdong‐Hong Kong‐Macao Joint Laboratory of Respiratory Infectious Diseases,Guangzhou Institutes of Biomedicine and Health,Chinese Academy of Sciences,Guangzhou,China China‐New Zealand Joint Laboratory on Biomedicine and Health,Guangzhou Institutes of Biomedicine and Health,Chinese Academy of Sciences,Guangzhou,China University of Chinese Academy of Sciences,Beijing,China State Key Laboratory of Respiratory Disease,Guangzhou Chest Hospital,Guangzhou,China
- 5. State Key Laboratory of Respiratory Disease,Guangzhou Institutes of Biomedicine and Health,Chinese Academy of Sciences,Guangzhou,China Guangdong‐Hong Kong‐Macao Joint Laboratory of Respiratory Infectious Diseases,Guangzhou Institutes of Biomedicine and Health,Chinese Academy of Sciences,Guangzhou,China China‐New Zealand Joint Laboratory on Biomedicine and Health,Guangzhou Institutes of Biomedicine and Health,Chinese Academy of Sciences,Guangzhou,China Institute of Physical Science and Information Technology,Anhui University,Hefei,China
- 6. State Key Laboratory of Respiratory Disease,Guangzhou Chest Hospital,Guangzhou,China Guangzhou National Laboratory,Guangzhou,China
- 7. State Key Laboratory of Respiratory Disease,Guangzhou Chest Hospital,Guangzhou,China
- 8. State Key Laboratory of Respiratory Disease,Guangzhou Institutes of Biomedicine and Health,Chinese Academy of Sciences,Guangzhou,China Guangdong‐Hong Kong‐Macao Joint Laboratory of Respiratory Infectious Diseases,Guangzhou Institutes of Biomedicine and Health,Chinese Academy of Sciences,Guangzhou,China China‐New Zealand Joint Laboratory on Biomedicine and Health,Guangzhou Institutes of Biomedicine and Health,Chinese Academy of Sciences,Guangzhou,China State Key Laboratory of Respiratory Disease,Guangzhou Chest Hospital,Guangzhou,China
- 9. State Key Laboratory of Respiratory Disease,Guangzhou Institutes of Biomedicine and Health,Chinese Academy of Sciences,Guangzhou,China Guangdong‐Hong Kong‐Macao Joint Laboratory of Respiratory Infectious Diseases,Guangzhou Institutes of Biomedicine and Health,Chinese Academy of Sciences,Guangzhou,China China‐New Zealand Joint Laboratory on Biomedicine and Health,Guangzhou Institutes of Biomedicine and Health,Chinese Academy of Sciences,Guangzhou,China University of Chinese Academy of Sciences,Beijing,China School of Basic Medical Sciences,Division of Life Science and Medicine,University of Science and Technology of China,Hefei,China State Key Laboratory of Respiratory Disease,Guangzhou Chest Hospital,Guangzhou,China Institute of Physical Science and Information Technology,Anhui University,Hefei,China Guangzhou National Laboratory,Guangzhou,China
- 折叠
摘要
关键词
CRISPR-Cas12a/DSB repair/Mycobacterium abscessus/NHEJ/NrgA分类
医药卫生引用本文复制引用
Sanshan Zeng,Yanan Ju,Md Shah Alam,Ziwen Lu,H.M.Adnan Hameed,Lijie Li,Xirong Tian,Cuiting Fang,Xiange Fang,Jie Ding,Xinyue Wang,Jinxing Hu,Shuai Wang,Tianyu Zhang..A CRISPR-nonhomologous end-joining-based strategy for rapid and efficient gene disruption in Mycobacterium abscessus[J].mLife,2025,4(2):P.169-180,12.基金项目
supported by the National Key R&D Program of China(2021YFA1300900 and 2022YFE0210600) （2021YFA1300900 and 2022YFE0210600）
the National Natural Science Foundation of China(21920102003) （21920102003）
the Guangdong Province Grant for Belt and Road Joint Laboratory(2022B1212050004) （2022B1212050004）
the State Key Lab of Respiratory Disease （）
the Postdoctoral Fellowship Program of CPSF(GZC20232688) （GZC20232688）
the Guangzhou Institute of Respiratory Diseases,First Affiliated Hospital of Guangzhou Medical University(SKLRD-Z-202301,SKLRD-Z-202412) （SKLRD-Z-202301,SKLRD-Z-202412）
the Guangzhou Science and Technology(2024A04J4273) （2024A04J4273）
the Chinese Academy of Sciences,President''s International Fellowship Initiative(2023VBC0015). （2023VBC0015）
