北华大学学报（自然科学版）2025，Vol.26Issue(4)：464-468,5.DOI:10.11713/j.issn.1009-4822.2025.04.007
牛磺酸通过调节iNOS活性抑制心肌成纤维细胞增殖的作用机制
Mechanism of Taurine in Inhibiting Myofibroblasts Proliferation by Regulating iNOS Activity
摘要
Abstract
Objective To investigate the inhibition of angiotensin Ⅱ(Ang Ⅱ)induced proliferation of rat pups myofibroblasts(myoFbs)by taurine(Tau)and to explore possible mechanisms involved.Methods Myocardial fibrosis(MF)model was established by using Ang Ⅱ to induce the proliferation of neonatal rat pups myoFbs in vitro.MTT was used to detect myoFbs proliferation;a hydroxyproline kit was used to measure collagen content;and immunocytochemical staining,laser confocal microscopy,and Western blot were used to detect the expression of inducible nitric oxide synthase(iNOS)in the cytoplasm of myoFbs.Results Compared to Ang Ⅱ group,Tau(30,60,120 mmol/L)significantly inhibited Ang Ⅱ induced myoFbs proliferation and collagen synthesis,with differences that were statistically significant(P＜0.05,P＜0.01)and exhibited dose-dependency.Immunocyto-chemical staining and laser confocal microscopy,combined with an image analysis system,showed that Tau could inhibit myoFbs proliferation by increasing the expression of iNOS in the cytoplasm of myoFbs and promoting protein synthesis,with differences that were statistically significant(P＜0.05,P＜0.01)when compared to Ang Ⅱ group.The results of Western blot analysis indicated that Tau could increase the expression of iNOS in the cytoplasm of myoFbs.Conclusion Tau inhibits MF by increasing the expression of iNOS in the cytoplasm of myoFbs,promoting NO synthesis,and inhibiting the proliferation of myoFbs and collagen synthesis.关键词
牛磺酸/血管紧张素Ⅱ/心肌成纤维细胞/诱导型一氧化氮合酶Key words
taurine/angiotensin Ⅱ/myofibroblasts/inducible nitric oxide synthase分类
医药卫生引用本文复制引用
李枫,马惠丽,乔超峰,张广超,鞠明,姜维华,王立英..牛磺酸通过调节iNOS活性抑制心肌成纤维细胞增殖的作用机制[J].北华大学学报（自然科学版）,2025,26(4):464-468,5.基金项目
吉林省自然科学基金面上项目(医学科学领域)(YDZJ202401015ZYTS). 本文是北华大学大学生创新创业训练项目(202410201042,202410201158,202310201104)研究成果的一部分,口腔医学院2021级黄鑫、2022级吕立群、2023级潘妍,临床医学院2022级潘思澄、李青怡、王子涵、朱晋辉等同学参与了本研究. （医学科学领域）
