分析测试学报2025，Vol.44Issue(7)：1299-1306,8.DOI:10.12452/j.fxcsxb.241029494
香豆素-铜(Ⅱ)配合物基一氧化氮荧光探针及其活体荧光成像研究
Coumarin-Copper(Ⅱ)-complex-based Fluorescent Probe for the Detection of NO and Its Application in in vivo Fluorescence Imaging
摘要
Abstract
Nitric oxide(NO)is one of three major signaling molecules,which involved in a great amount of physiological and pathological processes in biological systems.In addition,more and more evidences indicate that NO levels are closely related to several human health.Therefore,it is vital to develop a convenient and reliable method for NO detection in biological systems.In this paper,a new coumarin-Cu(Ⅱ)complex-based fluorescent probe YCY-Cu2+was successfully developed.In the presence of Cu2+,coumarin-based ligand YCY can specifically perform 1∶1 coordination with Cu2+to generate probe YCY-Cu2+.The strong green fluorescence of ligand YCY was quenched due to the paramagnetism of Cu2+.When NO was recognized by fluorescent probe YCY-Cu2+,the paramag-netic Cu2+was reduced to the diamagnetic Cu+and released from the complex,and the strong fluores-cent emitting N-nitroso compound(YCY-NO)are generated to achieve the specific recognition of NO.The structure of the fluorescent probe YCY-Cu2+and the recognition mechanism toward NO were confirmed by Job's analysis,high-resolution mass spectrometry(HRMS)and spectral titration analy-sis.The probe YCY-Cu2+has the advantages of high selectivity and sensitivity(LOD=97.3 nmol/L),wide range of pH applications(3.5-11.5)and low cytotoxicity.Most importantly,the probe has been successfully applied to the fluorescence imaging of NO in living cells and animals.关键词
荧光探针/香豆素/铜(Ⅱ)配合物/一氧化氮/荧光成像Key words
fluorescent probe/coumarin/copper(Ⅱ)-complex/nitric oxide/fluorescence imaging分类
化学化工引用本文复制引用
刘建华,伍玉林,尚主业,王月,张程,孟庆涛,张志强..香豆素-铜(Ⅱ)配合物基一氧化氮荧光探针及其活体荧光成像研究[J].分析测试学报,2025,44(7):1299-1306,8.基金项目
辽宁省教育厅面上项目(LJKMZ20220636) （LJKMZ20220636）
辽宁省教育厅青年项目(JYTQN2023246) （JYTQN2023246）
辽宁科技大学省级重点实验室开放课题基金(2024KFKT-10) （2024KFKT-10）
