分析测试学报2025，Vol.44Issue(7)：1362-1370,9.DOI:10.12452/j.fxcsxb.241031502
解吸电喷雾电离-高分辨质谱成像筛查分析蓝莓中小分子营养物质
Desorption Electrospray Ionization-High Resolution Mass Spectrometry Imaging Screening and Analysis of Small Molecule Nutrients in Blueberries
摘要
Abstract
A visual analysis method for in situ rapid screening of small molecular nutrients such as anthocyanins,anthocyanins and organic acids in blueberry was established by atmospheric pressure in situ desorption electrospray ionization-high resolution mass spectrometry imaging(DESI-MSI).By comparing the main instrumental analysis conditions such as spray solvent type,flow rate and spatial resolution,the optimal mass spectrometry imaging parameters were determined.In the positive ion mode,when methanol∶water(95∶5,by volume)with 0.2%formic acid and 0.2 μg/mL leucine enkephalin was used as the spray solvent,the highest ion peak signal intensity of the six anthocyanin standard samples at the concentration of 100 μg/L was the highest.Blueberries can be directly scanned and analyzed after frozen sections.Combined with the mass spectrometry information in the PubChem,a total of 4 anthocyanins,12 anthocyanins and 4 organic acids were identified,with a mass accuracy of≤5×10-6.The in-situ mass spectrometry imaging map intuitively showed the spatial distribution differences of small molecular nutrients in blueberry from different producing areas.The method was simple,rapid,accurate and reproducible,which provided effective technical support for the in-situ spatial distribution and metabolomics study of small molecular nutrients in blueberry.关键词
解吸电喷雾电离-高分辨质谱成像/蓝莓/花青素/原位/筛查分析/小分子营养物质Key words
desorption electrospray ionization-high resolution mass spectrometry imaging/blueber-ry/anthocyanins/in-situ/screening analysis/small molecule nutrients分类
化学化工引用本文复制引用
宋弦静,郭峰,刘斯文,赵虎,范金旭..解吸电喷雾电离-高分辨质谱成像筛查分析蓝莓中小分子营养物质[J].分析测试学报,2025,44(7):1362-1370,9.基金项目
国家自然科学基金项目(21976044) （21976044）
中国地质科学院基本科研业务费专项经费(JKYZD202418) （JKYZD202418）
