分析化学2025，Vol.53Issue(6)：864-874,11.DOI:10.19756/j.issn.0253-3820.241309
电化学传感技术用于端粒酶活性检测的研究进展
Research Progress on Electrochemical Sensing Techniques for Detection of Telomerase Activity
摘要
Abstract
The telomere structure in the cell nucleus is crucial for maintaining the stability and functions of chromosomes.Telomerase is a ribonucleoprotein reverse transcriptase,which catalyzes the elongation of telomeres using its own RNA as a template,thereby counteracting the shortening of telomeres caused by chromosome replication and cell division.Due to its overexpression in over 85％of malignant tumor cells,telomerase has emerged as a highly promising biomarker and a novel target for cancer therapy.In recent years,given the importance of precise quantification of telomerase activity in guiding medical diagnosis and treatment strategies,researchers have developed various high-performance telomerase detection techniques.Among these,electrochemical biosensing technique has cause much attention due to its high sensitivity,operational convenience,rapid response,and ease of miniaturization.This paper focused on the latest advances in electrochemical sensing technique for detection of telomerase activity,aiming to provide inspiration for designing novel telomerase activity detection strategies by elucidating three unique properties of telomerase primer extension products.关键词
化学传感/端粒酶活性/检测/评述Key words
Chemical sensing/Telomerase activity/Detection/Review引用本文复制引用
杨海堂,舒朋华,刘文林,马文博,杨子俊,邓志峰,张馨匀,卫伟..电化学传感技术用于端粒酶活性检测的研究进展[J].分析化学,2025,53(6):864-874,11.基金项目
国家自然科学基金项目(No.22304147)、河南省自然科学基金项目(No.232300421379)、河南省高等教育教学改革研究与实践项目(No.2024SJGLX0453)、河南省科技厅科技攻关项目(No.242102310503)和河南省高等学校重点科研项目(No.25B360001)资助. Supported by the National Natural Science Foundation of China(No.22304147),the Henan Natural Science Foundation of Youth Science Fund Project(No.232300421379),the Henan Higher Education Teaching Reform Research and Practice Project(No.2024SJGLX0453),the Project of Science and Technology Department of Henan Province(No.242102310503)and the Key Scientific Research Program in Universities of Henan Province(No.25B360001). （No.22304147）
