分析化学2025，Vol.53Issue(6)：903-913,11.DOI:10.19756/j.issn.0253-3820.241477
新型萘磺酰胺类开启型荧光探针的合成及用于草甘膦快速检测
Synthesis of A New Naphthalenesulfonamide-based"Turn-on"Fluorescent Probe for Rapid Detection of Glyphosate
摘要
Abstract
Widespread utilization of glyphosate has led to environmental residues,posing potential threats to ecological systems and human health.Traditional methods for detection of glyphosate are limited by specialized equipment and operational techniques,resulting in inefficient responses.Therefore,it is urgent to develop a convenient,sensitive and accurate detection method for detection of glyphosate.Herein,a new naphthalenesulfonamide-based"Turn-on"fluorescent probe was synthesized using 2-chloroaniline and dansyl chloride as raw materials through a one-step process,which showed a good linear relationship between the glyphosate concentration in concentration range of 0.003-70 μmol/L and the fluorescence intensity(R2=0.995),with a detection limit of 2.73 nmol/L(S/N=3).Analytical techniques such as nuclear magnetic resonance(NMR)spectroscopy and high-resolution mass spectrometry(HRMS)were used to investigate the interaction mechanism between the fluorescent probe and glyphosate.The results indicated that a nucleophilic substitution reaction occurred between the probe and the secondary amine(—NH—)of glyphosate,inducing a photoinduced electron transfer(PET)effect which enhanced the fluorescence intensity by 11.2 times.The probe showed good anti-interference ability towards coexisting metal ions,anions and pesticides in water.When applied to determination of glyphosate in the samples such as tap water,river water(Xiangxi River Reservoir),soil,soybeans,and corn,the spiking recoveries ranged from 94.7％to 109.9％,demonstrating the high accuracy and broad applicability of this detection method.A portable test strip based on this fluorescent probe was developed for rapid semi-quantitative analysis of glyphosate.The developed method was rapid,sensitive,and portable,providing theoretical and technical support for on-site measurement of environmental contaminants.关键词
萘磺酰胺类荧光探针/草甘膦/光致诱导电子转移/灵敏度/选择性Key words
Naphthalenesulfonamide-based fluorescent probes/Glyphosate/Photoinduced electron transfer/Sensitivity/Selectivity引用本文复制引用
赵蓉蓉,刘红林,黄应平,邓翠雯,李松岩,于水莲,陶茂胜,田义群,袁喜..新型萘磺酰胺类开启型荧光探针的合成及用于草甘膦快速检测[J].分析化学,2025,53(6):903-913,11.基金项目
国家自然科学基金项目(Nos.22406106,22476110,22136003)资助. Supported by the National Natural Science Foundation of China(Nos.22406106,22476110,22136003). （Nos.22406106,22476110,22136003）
