分析化学2025，Vol.53Issue(6)：914-923,10.DOI:10.19756/j.issn.0253-3820.251062
在有机溶剂体系中构建由RNA切割型脱氧核酶和G-四链体脱氧核酶串联的小分子传感系统
An RNA-cleaving DNAzyme and G-Quadruplex DNAzyme-Cascade System for Sensing of Small Molecules in Organic Cosolvent
摘要
Abstract
Detection of poor-water-soluble substances typically requires an organic solvent containing solution,and therefore,the biosensors that can operate in such conditions are highly promising for practical applications.However,most existing biosensors have been developed in aqueous solution and it is hard to work effectively in organic solvent systems.In this work,we uncovered that the G-quadruplex(G4)/hemin DNAzyme(G4HD)could be strongly reactivated by NH4 in organic solvents at concentration up to 30％,and the activity was significantly higher than that in aqueous solutions containing K+.Based on this discovery,a biosensing system was developed for small molecule in 40％methanol by coupling G4HD with our previously identified RNA-cleaving DNAzyme(E3).This system comprised an ATP-activated aptazyme that was made of E3 and a G4HD that was activated by the cleavage product of the aptazyme.By using a manmade pipette tip filter to separate the reaction products,the subsequent colorimetric reaction of the G4HD could be triggered.This biosensing system enabled the visualization of target molecules in organic cosolvents without any other instruments.The activable DNAzyme-cascade sensing system had potential in point-of-care detection of poor-water-soluble pollutants,thereby enhancing the practical values of functional nucleic acid-based biosensors in real-world detection conditions.关键词
脱氧核酶/适配体酶/有机溶剂/生物传感器/可视化Key words
DNAzyme/Aptazyme/Organic solvent/Biosensors/Visualization引用本文复制引用
李素慧,张帅奇,张毅波,郑权,常天俊..在有机溶剂体系中构建由RNA切割型脱氧核酶和G-四链体脱氧核酶串联的小分子传感系统[J].分析化学,2025,53(6):914-923,10.基金项目
国家自然科学基金项目(No.U1704241)和河南省科技攻关项目(No.242102321046)资助. Supported by the National Natural Science Foundation of China(No.U1704241)and the Science and Technology Research Project of Henan Province,China(No.242102321046). （No.U1704241）
