分析化学2025，Vol.53Issue(6)：924-933,中插6-中插15,20.DOI:10.19756/j.issn.0253-3820.241402
基于金属有机框架纳米片的比色传感器用于谷胱甘肽的高灵敏检测
Colorimetric Sensor for Sensitive Detection of Glutathione Based on Metal-Organic Framework Nanosheets
摘要
Abstract
Glutathione(GSH)is a tripeptide containing sulfhydryl groups,which has the function of antioxidant,detoxifying,and immune enhancing effects,and its expression is closely associated with many diseases such as cancer,etc.Therefore,it is of great significance for clinical diagnosis by developing a highly sensitive,simple,and rapid method for detecting GSH.Herein,two-dimensional(2D)Zn-TCPP(Fe)nanosheets employing Zn2+and Fe-bound tetrakis(4-carboxyphenyl)porphyrin ligands were obtained by a surfactant-assisted synthetic method.The 2D Zn-TCPP(Fe)nanosheets exhibited excellent peroxidase-like activity,which allowed the catalysis of the H2O2-initiated oxidation of colorless 3,3',5,5'-tetramethylbenzidine(TMB)to blue oxidized TMB(ox-TMB).In the presence of GSH,GSH underwent a reduction reaction with oxTMB,resulting in the blue color of the solution fading.The proposed colorimetric sensor exhibited favorable sensitivity for GSH in the linear range of 0.1-200 μmol/L with a limit of detection(S/N=3)of 0.042 μmol/L,indicating excellent selectivity.The developed sensor was successfully applied to detect GSH level in the human serum samples with recoveries of 93.0％-107.7％,showing satisfactory results.The developed colorimetric sensor provided a new approach for detecting GSH.关键词
比色传感器/谷胱甘肽检测/金属有机框架/纳米酶Key words
Colorimetric sensor/Glutathione detection/Metal-organic framework/Nanozyme引用本文复制引用
吴燕,冉婧蕾,刘芳,钟欢,陈锦杨..基于金属有机框架纳米片的比色传感器用于谷胱甘肽的高灵敏检测[J].分析化学,2025,53(6):924-933,中插6-中插15,20.基金项目
湖南省自然科学基金项目(No.2022JJ40364)、重庆市自然科学基金项目(No.CSTB2023NSCQ-MSX0229)、光电传感与生命分析教育部重点实验室开放课题项目(No.M2024-8)和广西药物精准检测与筛选重点实验室开放课题项目(No.GXPPDS202402)资助. Supported by the Natural Science Foundation of Hunan Province(No.2022JJ40364),the Natural Science Foundation Project of Chongqing(No.CSTB2023NSCQ-MSX0229),the Open Project Program of Key Laboratory of Optic-electric Sensing and Analytical Chemistry for Life Science,MOE(No.M2024-8),and the Guangxi Key Laboratory of Pharmaceutical Precision Detection and Screening(No.GXPPDS202402). （No.2022JJ40364）
