广西师范大学学报（自然科学版）2025，Vol.43Issue(4)：157-164,8.DOI:10.16088/j.issn.1001-6600.2024041401
氧化石墨烯介导的荧光强度和荧光偏振同时检测农药啶虫脒
Fluorescence Intensity and Polarization Method for Acetamiprid Detection Based on Graphene Oxide
摘要
Abstract
A fluorescence intensity and polarization method for simultaneous detection of pesticide acetamiprid was established based on the fluorescence quenching effect and mass amplification effect of graphene oxide(GO).When nucleic acid signal probe which labeled with fluorescent molecule FAM attached to the GO surface,a lower fluorescence intensity and a higher polarization signal was detected.After acetamiprid binds to its aptamer,the released complementary probe was hybridized with the signal probe to move it away from graphene,and then the enhanced fluorescence intensity and the reduced polarization signal are obtained.The relationship between the fluorescence intensity or polarization signal and different concentrations of acetamiprid was examined,and the conditions such as the target recognition time,GO concentration and reaction time were optimized.Under the optimized experimental conditions,the fluorescence polarization detection method(LOD=5 nmol/L)had a lower detection limit than the fluorescence intensity method(LOD=50 nmol/L),while the fluorescence intensity method was more stable.This method showed good selectivity and achieved spiked recovery analysis of acetamiprid in celery leaves and lake water.关键词
啶虫脒/氧化石墨烯/荧光分析/荧光偏振/荧光猝灭Key words
acetamiprid/graphene oxide/fluorescence analysis/fluorescence polarization/fluorescence quenching分类
化学化工引用本文复制引用
尹南竹,黄茜,赵晶瑾..氧化石墨烯介导的荧光强度和荧光偏振同时检测农药啶虫脒[J].广西师范大学学报（自然科学版）,2025,43(4):157-164,8.基金项目
国家自然科学基金(22376040,21966009) （22376040,21966009）
