河北农业大学学报2025，Vol.48Issue(3)：1-10,57,11.DOI:10.13320/j.cnki.jauh.2025.0033
陆地棉GhCER2通过调节蜡质合成响应干旱胁迫的功能研究
Study on the function of GhCER2 in response to drought stress by regulating wax synthesis in Gossypium hirsutum
摘要
Abstract
The ECERIFERUM 2(CER2),belonging to the family of fatty acid hydroxylases,is involved in the extension process of very long chain fatty acids(VLCFAs)C28 during the wax synthesis process.In order to reveal the function of GhCER2 in regulating the drought tolerance of cotton,this study utilized bioinformatics,physiology and molecular biology,combined with methods such as scanning electron microscopy,to systematically analyze and verify the molecular characteristics and biological functions of GhCER2.It was found that there were two homologous genes of GhCER2 located on the At and Dt subgenomes in upland cotton,and the evolutionary conservation of CER2 was revealed.The GhCER2 promoter region contained cis-acting elements related to drought and hormonal signal responses,and its transcription was induced by drought stress and ABA signals.Silencing GhCER2 resulted in a decline in the drought resistance of cotton seedlings and an imbalance in the antioxidant system.Further electron microscopy observations of the seedlings of the GhCER2 silencing plants revealed an accumulation of waxy crystals on the epiderm leaves,an accelerated leaching and water loss rate of chlorophyll,and a decrease in the water retention of the leaves.Under drought conditions,GhCER2 also regulated the transpiration rate of leaves by changing the stomatal state.This study revealed the regulatory mechanism that GhCER2 affeded the drought tolerance of cotton by regulating the accumulation of waxy matter on the leaf epidermis and the stomatal state.关键词
GhCER2/蜡质/气孔调控/干旱胁迫Key words
GhCER2/wax/stomatal regulation/drought stress分类
农业科技引用本文复制引用
吴若兵,王亦凡,包蕊,白志英,李存东,张科..陆地棉GhCER2通过调节蜡质合成响应干旱胁迫的功能研究[J].河北农业大学学报,2025,48(3):1-10,57,11.基金项目
国家自然科学基金项目(32372036) （32372036）
河北省自然科学基金项目(C2023204302 （）
C2022204036) （）
华北作物改良与调控国家重点实验室项目(NCCIR2023ZZ-7) （NCCIR2023ZZ-7）
河北省科技计划项目(23567601H). （23567601H）
