河北农业大学学报2025，Vol.48Issue(3)：11-21,11.DOI:10.13320/j.cnki.jauh.2025.0034
m7G甲基转移酶基因GhTRM8a/82调控棉花株高发育的研究
A Cotton m7G methyltransferase core complex GhTRM8a/82 regulate cotton plant height development
摘要
Abstract
m7G-methyladenosine(m7G),as one of the ubiquitous tRNAs modifications from bacteria to humans,plays an important role in regulation of gene expression.The core complex of m7G methyltransferase consists of METTL1 and its cofactor WDR4,but the role of their homologous proteins in plant growth and development has been rarely reported.In this study,we identified GhTRM8a and GhTRM82,the members of core complex of m7G methyltransferase in upland cotton,and found that TRM8a and TRM82 were highly conserved during plant evolution.GhTRM8a and GhTRM82 were expressed in various tissues,and their encoded proteins were localized in the nucleus and can interact to form heterodimers.Virus-induced gene silencing(VIGS)of GhTRM8a and GhTRM82 expression resulted in shorter stems and significantly shorter cotton plants.Stem longitudinal paraffin sections showed that the silencing of GhTRM8a and GhTRM82 significantly shortened the length of stem cells,indicating that GhTRM8a/GhTRM82 regulated the development of cotton plant height by affecting stem cell elongation.The qRT-PCR experimental results demonstrated that GhTRM8a and GhTRM82 regulate cotton stem development by influencing the expression of genes associated with gibberellin(GA)biosynthesis,as well as auxin and strigolactone pathways.In summary,GhTRM8a and GhTRM82 interacted to form a protein complex,which modulated cotton stem development through regulating GA biosynthesis.This study provides mechanistic insights into the regulation of plant architecture development in cotton.关键词
棉花/株高发育/m7G甲基转移酶/GhTRM8a/GhTRM82/GA合成Key words
cotton/plant height development/m7G methyltransferase/GhTRM8a/GhTRM82/GA synthesis分类
农业科技引用本文复制引用
魏修振,门婧婕,张慧,余志喜,张经博,赵惠新,黄耿青..m7G甲基转移酶基因GhTRM8a/82调控棉花株高发育的研究[J].河北农业大学学报,2025,48(3):11-21,11.基金项目
新疆维吾尔自治区杰出青年科学基金项目(2021D01E17) （2021D01E17）
国家自然科学基金项目(32160465) （32160465）
棉花生物育种与综合利用全国重点实验室开放课题项目(CB2024A31). （CB2024A31）
