河北农业大学学报2025，Vol.48Issue(3)：58-64,7.DOI:10.13320/j.cnki.jauh.2025.0039
发根农杆菌介导的黄瓜CsPIP2;4过表达复合植株获得及耐盐性鉴定
Obtaining composite plants with overexpression of CsPIP2;4 in cucumber mediated by Agrobacterium rhizogenes and identification of their salt tolerance
摘要
Abstract
To elucidate the function of CsPIP2;4 in salt stress tolerance of cucumber,this study introduced the empty plasmid of the pCAMBIA2300s and the pCAMBIA2300s:CsPIP2;4 plasmid into Agrobacterium rhizogenes strain K599,respectively.Composite cucumber plants overexpressing CsPIP2;4(OP)and empty vector-transformed(EV)plants were generated via hypocotyl injection.Morphological characteristics and physiological indexes,including malondialdehyde(MDA)content,soluble sugar and protein levels,and superoxide dismutase(SOD)activity,were comparatively analyzed under salt stress.Results demonstrated that OP composite plants exhibited superior growth phenotypes compared to EV controls under salt stress,with reduced leaf chlorosis and marginal scorching symptoms,as well as more developed root systems.Concurrently,OP plants showed decreased MDA content and SOD activity in leaves,alongside elevated soluble protein and sugar levels.All measured parameters displayed significant or highly significant differences between OP and EV plants after stress treatment for 7 days.These findings indicated that CsPIP2;4 positively regulated salt tolerance of cucumber,which was closely associated with its promotion of osmotic adjustment substances accumulation,particularly soluble sugars and proteins.关键词
黄瓜/CsPIP2/4/发根农杆菌/复合植株/盐胁迫Key words
cucumber/CsPIP2/4/Agrobacterium rhizogenes/composite plant/salt stress分类
农业科技引用本文复制引用
陈思,陈梅,赖梦霞,杜长霞,樊怀福..发根农杆菌介导的黄瓜CsPIP2;4过表达复合植株获得及耐盐性鉴定[J].河北农业大学学报,2025,48(3):58-64,7.基金项目
国家自然科学基金项目(32272700) （32272700）
浙江省基础公益研究计划项目(LGN22C150007) （LGN22C150007）
浙江省自然科学基金项目(LY23C150005). （LY23C150005）
