华东师范大学学报（自然科学版）Issue(4)：104-113,10.DOI:10.3969/j.issn.1000-5641.2025.04.011
凡纳滨对虾肠道及其养殖环境的细菌群落结构研究
Study on bacterial community structure in the intestine of Litopenaeus vannamei and its cultivation environment
摘要
Abstract
The aim of this study was to compare the bacterial community structure of Litopenaeus vannamei in different cultivation environments.An Illumina MiSeq high-throughput sequencing-based method was used for detecting the 16S rRNA gene of the bacterial community in the intestine of L.vannamei and water and sediment samples from cultivation ponds.The results showed that the bacterial community in the intestine of L.vannamei and in the cultivation environment included 62 phyla,175 classes,381 orders,631 families,1 141 genera,and 2 035 species.Proteobacteria,Actinobacteriota,and Chloroflexi were the dominant bacterial phyla in the intestine of L.vannamei,with average percentages of 33.67%,25.33%,and 12.77%,respectively.Proteobacteria,Chloroflexi,and Firmicutes were the dominant bacteria in the sediments,accounting for 28.33%,17.33%,and 11.13%,respectively.The dominant bacteria in the water were Actinobacteria,Cyanobacteria,and Proteobacteria,with average percentages of 29.33%,27.0%,and 21.33%,respectively.The abundance-based coverage estimator(ACE)and Chao diversity index of bacterial community in the intestine of L.vannamei were higher than those in the water,while lower than those in the sediments.The bacterial community richness in the intestine of L.vannamei was similar to that in water,but higher than that in the sediments.The Shannon index of the bacterial community in the intestine of L.vannamei was higher than that in the sediments,but lower than that in water.The Simpson index of the bacterial community in the intestine of L.vannamei was higher than that in the sediments but lower than that in the water.Nevertheless,some dominant bacterial species were similar in the intestine of L.vannamei and in the cultivation environment.At the operational taxonomic unit(OTU)level,1 492 identical units were detected in the intestines of L.vannamei and sediments,and 588 identical units were detected in the intestine of L.vannamei and water.Both cluster and principal coordinate analyses showed that the bacterial community in the intestine of L.vannamei was relatively similar to that in the sediments.This study explored the relationship between the bacterial community in the intestine of L.vannamei and its cultivation environment,providing valuable data for the scientific use of environmental probiotics,quality and production,and disease and epidemic prevention.关键词
凡纳滨对虾/高通量测序/细菌群落/16S rRNAKey words
Litopenaeus vannamei/high-throughput sequencing/bacterial community structure/16S rRNA分类
农业科技引用本文复制引用
金沁,邱楚雯,袁新程..凡纳滨对虾肠道及其养殖环境的细菌群落结构研究[J].华东师范大学学报（自然科学版）,2025,(4):104-113,10.基金项目
上海市现代农业产业技术体系(沪农科产字[2022]第5号) （沪农科产字[2022]第5号）
