核农学报2025，Vol.39Issue(8)：1754-1762,9.DOI:10.11869/j.issn.1000-8551.2025.08.1754
黄精发酵液的抗氧化、抗炎和美白潜力研究
Study on the Antioxidant,Anti-Inflammatory and Whitening Potential of Polygonatum sibiricum Red. Fermented Broth
摘要
Abstract
To study the feasibility of Polygonatum sibiricum Red.fermented broth as a functional raw material for cosmetics,Lactic acid bacteria,yeast,bacillus subtilis,and mixed bacteria were used to carry out liquid fermentation of Polygonatum sibiricum Red.,respectively.The contents of polysaccharides,total saponins,total polyphenols,and total flavonoids in the Polygonatum sibiricum Red.fermentation broth were quantified by spectrophotometry.The antioxidant,anti-inflammatory,whitening and safety properties of the fermentation broth were evaluated by measuring the ability to scavenge 1,1-diphenyl-2-picryl-hydrazyl(DPPH),2,2'-azinobis-(3-ethylbenzthiazoline-6-sulphonate)(ABTS),and hydroxyl radicals,the toxicity of fementation to human immortalized keratinocytes(HaCaT)cells,the effect on the levels of inflammatory factors tumor necrosis factor-alpha(TNF-α)and interleukin 6(IL-6)in HaCaT cells,and the effect on tyrosinase activity in intracellularly and extracellularly.The results indicated that the levels of polyphenols and flavonoids in the fermentation broth of Polygonatum sibiricum Red.were significantly higher than that of unfermented samples.Notably,the samples fermented with mixed bacteria exhibited the highest concents of these two types of active compounds,which increased by 33.21％and 57.94％,respectively.The scavenging capabilities of the Polygonatum sibiricum Red.fermentation broth against DPPH and ABTS free radicals were stronger than that of the unfermented Polygonatum sibiricum Red.extract.The scavenging rates of DPPH and ABTS free radicals by mixed bacteria fermentation broth were 87.65％and 72.5％,respectively.Polygonatum sibiricum Red.fermentation broth was superior to unfermented broth in terms of safety and inhibition of intracellular inflammatory factors TNF-α and IL-6 on HaCaT.The Polygonatum sibiricum Red.fermentation broth with mixed bacteria showed good inhibitory effect on tyrosinase,achieving the inhibition rate of 92.39％in vitro and an intracellular inhibition rate of 65.50％at a concentration of 100 mg·mL-1.In conclusion,Polygonatum sibiricum Red.fermentation broth has good antioxidant,anti-inflammatory and whitening,which provides experimental basis for its application as a functional raw material in cosmetics.关键词
黄精发酵液/活性成分/抗自由基/抗炎因子/抗酪氨酸酶Key words
Polygonatum sibiricum Red. fermented broth/active ingredient/anti-free radical/anti-inflammatory factor/anti-tyrosinase引用本文复制引用
王丹丹,奚林芝,刘畅,王盛东,刘利萍..黄精发酵液的抗氧化、抗炎和美白潜力研究[J].核农学报,2025,39(8):1754-1762,9.基金项目
宁波公益(重点)科技计划项目(2022S186),浙江省"生物工程"一流学科学生创新项目(CX2023012) （重点）
